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- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
BASU RaPID
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
BASU RaPID / BASU RaPID 璐ㄧ矑
BASU RaPID plasmid
PVT14054 2ug
BASU RaPID plasmid Search name
BASU RaPID plasmid ,BASU RaPID plasmid vector,BASU RaPID plasmid plasmid,BASU RaPID plasmid map
BASU RaPID plasmid Information
Bacterial Resistance(s): Amp
Screening:
Growth Strain(s): Stbl3
Growth Condition: LB/37 Centigrade
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文献和实验A Rapid Plasmid Purification Method for Dideoxy Sequencing
. Traditionally, sequencing was performed on templates produced by single-stranded phage Ml3 (2 ,3 ), or phagmid (4 ). Sequencing plasmid DNA has the advantage of eliminating the need to subclone fragments into M13, and also enables both strands to be sequenced
The Rapid Boiling Method for Small-Scale Preparation of Plasmid DNA
The classic alternative to the alkaline lysis method for plasmid DNA preparation (1; see Chapters 31 and 33 ) is that of Holmes and Quigley (2 ), and is commonly known as the rapid boiling method. This method is based on exactly
selection in Escherichia coli that allows rapid profiling of nucleotide substitutions within a target site of given endonucleases. The selection utilizes a toxic plasmid (pTox) that encodes a DNA gyrase toxin in addition to the endonuclease target site
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