pAdVAntage
Catalog No. PVT11020
Packing 2ug
pAdVAntage Information
Function Mammal plasmids
Promoter: Lac
Replicon: pUC ori
Plasmid classification: mammalian cells, protein overexpression vectors
Plasmid size: 4392bp
Prokaryotic resistance: Amp
Cloned strain: DH5 alpha
Culture conditions: 37 centigrade, aerobic LB
Expression host: mammalian cells
Induction mode: no induction, instantaneous expression
5'sequencing primers: M13R:CAGGAAACAGCTATGACC
3'sequencing primers: primers designed according to sequence
pAdVAntage Description
The use of pAdVAntage vectors were transfected into mammalian cells can promote the initiation of translation to enhance the instantaneous protein expression in many cell types. Transfection of mammalian cells with expression vector often leads to the expression of the target protein. The double stranded RNA (dsRNA), which is produced in the transfection process, is thought to activate the dsRNA- activation inhibitor (DAI), which is one of the many kinds of enzymes involved in the host cell antiviral defense system. DAI phosphorylation of translation initiation factor eIF-2, termination of translation and generation of protein. However, with the pAdVAntage vector were transfected into DAI in the process of translation inhibition by RNA polymerase III generated adeno-associated virus type I (RNA VAI RNA) offset. RNA DAI combined with VAI to prevent its activation, so as to complete the translation and protein expression.
pAdVAntage Sequence
LOCUS Exported 4392 bp ds-DNA circular SYN 15-JUL-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pAdVAntage
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4392)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Tuesday, August 30, 2016 from SnapGene Viewer 3.1.4
FEATURES Location/Qualifiers
source 1..4392
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1743..1759)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1767..1783
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1791..1821)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1836..1857
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2145..2733)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2904..3764)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3765..3869)
/gene="bla"
/note="AmpR promoter"
primer_bind 4343..4359
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 gtcgacagaa gcaccatgtc cttgggtccg gcctgctgaa tgcgcaggcg gtcggccatg
61 ccccaggctt cgttttgaca tcggcgcagg tctttgtagt agtcttgcat gagcctttct
121 accggcactt cttcttctcc ttcctcttgt cctgcatctc ttgcatctat cgctacggcg
181 gcggcggagt ttggccgtag gtggcgccct cttcctccca tgcgtgtgac cccgaagccc
241 ctcatcggct gaagcagggc caggtcggcg acaacgcgct cggctaatat ggcctgctgc
301 acctgcgtga gggtagactg gaagtcatcc atgtccacaa agcggtggta tgcgcccgtg
361 ttgatggtgt aagtgcagtt ggccataacg gaccagttaa cggtctggtg acccggctgc
421 gagagctcgg tgtacctgag acgcgagtaa gcccttgagt caaagacgta gtcgttgcaa
481 gtccgcacca ggtactgata tcccaccaaa aagtgcggcg gcggctggcg gtagaggggc
541 cagcgtaggg tggccggggc tccgggggcg aggtcttcca acataaggcg atgatatccg
601 tagatgtacc tggacatcca ggtgatgccg gcggcggtgg tggaggcgcg cggaaagtcg
661 cggacgcggt tccagatgtt gcgcagcggc aaaaagtgct ccatggtcgg gacgctctgg
721 ccggtgaggc gtgcgcagtc gttgacgctc tagaccgtgc aaaaggagag cctgtaagcg
781 ggcactcttc cgtggtctgg tggataaatt cgcaagggta tcatggcgga cgaccggggt
841 tcgaaccccg gatccggccg tccgccgtga tccatgcggt taccgcccgc gtgtcgaacc
901 caggtgtgcg acgtcagaca acgggggagc gctccttttg gcttccttcc aggcgcggcg
961 gctgctgcgc tagctttttt ggccactggc cgcgcgcggc gtaagcggtt aggctggaaa
1021 gcgaaagcat taagtggctc gctccctgta gccggagggt tattttccaa gggttgagtc
1081 gcaggacccc cggttcgagt ctcgggccgg ccggactgcg gcgaacgggg gtttgcctcc
1141 ccgtcatgca agaccccgct tgcaaattcc tccggaaaca gggacgagcc ccttttttgc
1201 ttttcccaga tgcatccggt gctgcggcag atgcgccccc ctcctcagca gcggcaagag
1261 caagagcagc ggcagacatg cagggcaccc tccccttctc ctaccgcgtc aggaggggca
1321 acatccgcgg ctgacgcggc ggcagatggt gattacgaac ccccgcggcg ccgggcccgg
1381 cactacctgg acttggagga gggcgagggc ctggcgcggc taggagcgcc ctctcctgag
1441 cgacacccaa gggtgcagct gaagcgtgac acgcgcgagg cgtacgtgcc gcggcagaac
1501 ctgtttcgcg accgcgaggg agaggagccc gaggagatgc gggatcgaaa gttccacgca
1561 gggcgcgagt tgcggcatgg cctgaaccgc gagcggttgc tgcgcgagga ggactttgag
1621 cccgacgcgc ggaccgggat tagtcccgcg cgcgcacacg tggcggccgc cgacctggta
1681 accgcgtacg agcagacggt gaaccaggag attaactttc aaaaaagctt ggcgtaatca
1741 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
1801 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
1861 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
1921 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
1981 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
2041 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
2101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
2161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
2221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
2281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
2341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
2401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
2461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
2521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
文献和实验
技术资料
