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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pSilencer 1.0-U6
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pSilencer 1.0-U6
Catalog No. PVT10866
Packing 2ug
pSilencer 1.0-U6 Information
Function Mammal Editing plasmids
Promoter: U6
Prokaryotic resistance: ampicillin Amp
Cloned strain: Escherichia coli DH5 alpha
Culture conditions: 37 degrees centigrade
pSilencer 1.0-U6 Description
PSilencer 1.0-U6 is a lactating RNA expression plasmid.
pSilencer 1.0-U6 Sequence
LOCUS Exported 3292 bp ds-DNA circular SYN 20-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..3292
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 675..988
/label=U6 promoter
/note="RNA polymerase III promoter for mouse U6 snRNA (Das
et al., 1988)"
misc_feature 990..1091
/label=MCS
rep_origin complement(1545..2133)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2304..3164)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3165..3269)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg
661 ctctagaact agtggatccg acgccgccat ctctaggccc gcgccggccc cctcgcacag
721 acttgtggga gaagctcggc tactcccctg ccccggttaa tttgcatata atatttccta
781 gtaactatag aggcttaatg tgcgataaaa gacagataat ctgttctttt taatactagc
841 tacattttac atgataggct tggatttcta taagagatac aaatactaaa ttattatttt
901 aaaaaacagc acaaaaggaa actcacccta actgtaaagt aattgtgtgt tttgagacta
961 taaatatccc ttggagaaaa gccttgtttg ggccccccct cgaggtcgac ggtatcgata
1021 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
1081 cggtggagct ccagcttttg ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca
1141 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
1201 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
1261 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
1321 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
1381 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
1441 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
1501 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
1561 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
1621 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
1681 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
1741 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
1801 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
1861 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
1921 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
1981 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
2041 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
2101 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
2161 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
2221 aggannttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
2281 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
2341 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
2401 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
2461 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
2521 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
2581 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
2641 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
2701 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
2761 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
2821 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
2881 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
2941 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
3001 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
3061 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
3121 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
3181 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
3241 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc ac
//
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文献和实验该产品被引用文献
Structural basis for the interaction of the chaperone Cbp3 with newly synthesized cytochrome b during mitochondrial respiratory chain assembly
Mama Ndi1, Geoffrey Masuyer1, Hannah Dawitz1, Andreas Carlstr?m1, Mirco Michel1, Arne Elofsson2, Mikaela Rapp1, P?l Stenmark3 and Martin Ott3*
First Published on Journal of biological chemistry
September 19, 2019
doi: 10.1074/jbc.RA119.010483
jbc.RA119.010483.
相关实验
U6 vector, nucleotide overhangs to the EcoR I and Apa I restriction sites are added to the 5' and 3' end of the DNA oligonucleotides, respectively (Figure 2). In contrast, for cloning into the pSilencer 2.0-U6, 2.1-U6, 3.0-H1, or 3.1-H1 vectors
a 9-nucleotide spacer (TTCAAGAGA), although other spacers can be designed. 5-6 T's are added to the 3' end of the oligonucleotide. In addition, for cloning into the pSilencer 1.0-U6 vector, nucleotide overhangs to the Eco R I and Apa I restriction sites
PREPARATION OF PLASMID DNA FOR SEQUENCING
. Incubate at 37℃ overnight with vigorous shaking. Follow the Plasmid Quick Prep protocol through the potassium acetate step to isolate plasmid DNA. Add DNAse-free RNAse to 50µg/ml, incubate, 37℃ from 10-30 minutes. Phenol extract the solution, saving
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pSilencer 1.0-U6 Plasmid
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