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- LSM BIO
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- PVT10558
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pSXneo 270 (T2AG3)
- 库存：
50
- 供应商：
LSMBIO
- 规格：
2ug
pSXneo 270(T2AG3)
Catalog No. PVT10558
Packing 2ug
pSXneo 270 (T2AG3) Information
> SP6 5'-ATTTAGGTGACACTATAG-3'
ACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCC
TAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAAC
CCTAACCCTAACCCCAACCCCAACCCCAACCCCAACCCTAACCCTAACCCTAACCCCAACCGCAACCCTA
ACCCCAACCCCAACCCCAACCCTAACCCCAACCGCAACCCCAACCCCAACCCGAAGCCTAACCCTAACCC
TAACCCTAACCCGAACCCCAACC
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文献和实验BACs, PACs, P1s and Plasmid Isolation Protocol (standard protocol)
mixing as this will shear chromosomal DNA and lower plasmid purity. The lysate should appear viscous. Do not allow the lysis reaction to proceed more than 5 min. (Store Buffer T2 tightly capped when not in use). 5. Add 200 ul of Chilled Buffer T
BACs, PACs, P1s and Plasmid Isolation Protocol (For low copy number)
and discard. 3. Resuspend the bacterial pellet by adding 260 ul of Buffer T1/RNase A solution, and vortexing. Complete resuspension of cell pellet is vital for obtaining good yields. 4. Add 260 ul of Buffer T2 and gently mix by inverting 5-10 times
primers and ligated into a mammalian expression vector under the control of a CMV promoter. Identification of a plasmid possessing the open reading frame of the paraoxonase-1 gene was carried out by PCR analysis using gene specific primers. Confirmation
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