- $216
- LSM BIO
- 进口
- PVT80842
- 2025年07月12日
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- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
PiggyBac-EF1a-IMS-GFP11-FLAG-EF1a-Puro Plasmid
- 库存：
100
- 供应商：
LSM BIO
- 规格：
2ug
PiggyBac-EF1a-IMS-GFP11-FLAG-EF1a-Puro Plasmid
Catalog No.: PVT80842
PiggyBac-EF1a-IMS-GFP11-FLAG-EF1a-Puro Plasmid
Alias:-
Host: Mammalian cells
Use(s): Protein expression,transposition
Fragment Type: ORF
Fragmented species: soybean
Prokaryotic resistance: Amp
Screening Markers: Puro
Fluorescence labeling: Green
Promoter: EF1a
Competent cells: Stbl3
Temperature: 37Celsius
Caution:
1. This product is FOR RESEARCH USE ONLY!
2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3. Shipping temperature is 2-8 degrees centigrade.
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文献和实验naj2007 请教一下各位老师，在构建过表达载体的时候，VEGF和GFP是不是不可以做成融合蛋白，但是如果用不同的启动子的话，很容易丢失荧光，有没有什么解决方法。 zhujoker 可以做成融合蛋白的，即使是使用不同的启动子的话荧光也不那么容易丢失的，大不了你将荧光的启动子换成强的如CMV，EF1a之类。 kinguangjun 或者你可以用CMV-VEGF-IRES-GFP来做
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
volume of at least 1 ml. Apply the sample to a previously equilibrated QIAGEN-tip 20. Note: To compare the amount of plasmid in the culture with amount obtained after purification, remove a comparable aliquot (11%) of the final purified sample and quantify
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