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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pUC57s-Lox-m (356-1236)
- 库存:
50
- 供应商:
LSMBIO
- 规格:
2ug
pUC57s-Lox-m(356-1236)
Catalog No. PVT10431
Packing 2ug
Function DNA Cloning plasmid
Resistance Amp
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer ?
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文献和实验PREPARATION OF PLASMID DNA FOR SEQUENCING
The following protocol is based on our modifications of R. Kraft, J. Tardiff, K. S. Krauter, and L. A. Leinwand. Biotechn . 6(6):544-545, 1988. Inoculate 2-5 ml of L broth containing the appropriate antibiotic from a single bacterial colony
isopropanol and mixed.Spin 10-15 min.Discard supernatant and wash pellet with 1 ml of 100% EtOH.dry pellets and resuspend in 50 µl H2O or TE.Buffer A:50 mM TrisHCl pH 8.0,10 mM EDTA,100 ug/ml RNase A. Buffer B:200 mM NaOH,1% SDS. 7.5 M NH4Ac (57.75
as possible as sometimes transposon 'hot-spots' into it. Select transformants on LB Kan40. Transform target plasmid into competent cells of R1236/B211. Select on LB Kan40 Cm34. Transfer F::mTn-3xHA/lacZ
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