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- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pT7-His-Nrf2
- 库存:
10
- 供应商:
LSMBIO
- 规格:
质粒干粉
pT7-His-Nrf2
Catalog No. PVT10132
Packing 2ug
Function ORF expression plasmid
Resistance Kan
Screen /
Strain DH5alpha
Culture temperature 37degrees centigrade
Replicon
Copy
Promoter
Induction
Forward primer
Reverse primer ?
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文献和实验Reaction Mix: It is again convenient to make up a master mix to be aliquotted out to amplify all samples, if they are to use the same primers. If not, modify master mix by simply leaving out primers. Total volume: 50ul / rxn x N + 5-10
Role of Nrf2-Dependent ARE-Driven Antioxidant Pathway in Neuroprotection
The promoter regions of many detoxification enzymes contain a cis -acting enhancer known as the antioxidant response element (ARE). NF-E2-related factor 2 (Nrf2) is considered as one of the major transcription factors
This protocol is for Mini (up to 20 µg) preparations of high-copy plasmid DNA from cultures of E. coli . Important notes before starting New users are strongly recommended to read Appendix A (page 21) at the end
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