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- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pET-12b
- 库存：
10
- 供应商：
LSMBIO
- 规格：
质粒干粉
pET-12b
Search name
pET-12b,Plasmid pET-12b,pET-12b vector
pET-12b Information
Plasmid type: Escherichia coli expression vector
Expression level: high
Cloning methods: polyclonal sites, restrictive endonucleases
Carrier size: 4673bp
5'sequencing primers: T7/ lacI
Sequence of 5'sequencing primers: 5'-TAATACGACTCACTATAGGG-3'
Carrier label: ompT
Carrier resistance: Ampicillin
Cloned strain: DH5a
Expression strain: BL21 series
Remarks:
PET-12a, B, C only read the frame (read code box) different, each other 1 bp.
Product directory number: -
Stability: stable expression
Composition type: inducible type (IPTG)
Virus / non virus: non virus
pET-12b Description
| Feature Name | Start | End | |
|---|---|---|---|
| tet (w/ gaps) | 86 | 1586 | |
| T7_terminator | 507 | 379 | |
| T7_Terminal_primer | 447 | 465 | |
| T7_transl_en_RBS | 605 | 589 | |
| T7_promoter | 660 | 642 | |
| pBRrevBam_primer | 763 | 744 | |
| ROP | 2225 | 2416 | |
| pGEX_3_primer | 2432 | 2410 | |
| Ampicillin | 4465 | 3605 | |
| AmpR_promoter | 4535 | 4507 |
| ORF | Start | End | |
|---|---|---|---|
| ORF frame 2 | 797 | 1384 | |
| ORF frame 1 | 1391 | 906 | |
| ORF frame 3 | 969 | 1586 | |
| ORF frame 2 | 4465 | 3605 |
| Enzyme Name | Cut |
|---|---|
| ClaI | 24 |
| HindIII | 29 |
| NheI | 229 |
| BamHI | 510 |
| SalI | 516 |
| NdeI | 579 |
| XbaI | 617 |
| BglII | 675 |
| EagI | 1249 |
| MscI | 1756 |
| PvuII | 2376 |
| PstI | 3923 |
| AatII | 4600 |
| EcoRI | 4671 |
| pET-Vectors -protocol | ||||||
| Vector Name | Promoter | Resistance | C-tag | N-tag | Protease cleavage site | Application |
| pET-3a,b,c | T7 | Ampicillin | 閵嗏偓 | T7路Tag | 閵嗏偓 | Constituent expression |
| pET-11a,b,c,d | T7 lac | Ampicillin | 閵嗏偓 | T7路Tag | 閵嗏偓 | 閵嗏偓 |
| pET-12a | T7 lac | Ampicillin | 閵嗏偓 | ompT | 閵嗏偓 | 閵嗏偓 |
| pET-14b | T7 lac | Ampicillin | 閵嗏偓 | His路Tag | Thrombin | 閵嗏偓 |
| pET-15b | T7 lac | Ampicillin | 閵嗏偓 | His路Tag | Thrombin | 閵嗏偓 |
| pET-16b | T7 lac | Ampicillin | 閵嗏偓 | His路Tag | Factor Xa | 閵嗏偓 |
| pET-17b | T7 lac | Ampicillin | 閵嗏偓 | T7路Tag | 閵嗏偓 | 閵嗏偓 |
| pET-19b | T7 lac | Ampicillin | 閵嗏偓 | His路Tag | Enterokinas | 閵嗏偓 |
| pET-20b | T7 lac | Ampicillin | His路Tag | pelB signal peptide | 閵嗏偓 | secretory expression |
| pET-21a,b,c,d | T7 lac | Ampicillin | His路Tag | T7路Tag | 閵嗏偓 | 閵嗏偓 |
| pET-22b | T7 lac | Ampicillin | His路Tag | pelB signal peptide | 閵嗏偓 | secretory expression |
| pET-23a,b,c,d | T7 | Ampicillin | His路Tag | T7路Tag | 閵嗏偓 | Constituent expression |
| pET-24a,b,c,d | T7 lac | Kanamycin | His路Tag | T7路Tag | 閵嗏偓 | 閵嗏偓 |
| pET-25b | T7 lac | Ampicillin | HSV路Tag,His路Tag | pelB signal peptide | 閵嗏偓 | secretory expression |
| pET-26b | T7 lac | Kanamycin | His路Tag | pelB signal peptide | 閵嗏偓 | secretory expression |
| pET-27b | T7 lac | Kanamycin | HSV路Tag,His路Tag | pelB signal peptide | 閵嗏偓 | secretory expression |
| pET-28a,b,c | T7 lac | Kanamycin | His路Tag | His路Tag,T7路Tag | Thrombin | 閵嗏偓 |
| pET-29a | T7 lac | Kanamycin | His路Tag | S路Tag | Thrombin | 閵嗏偓 |
| pET-30a,b,c | T7 lac | Kanamycin | His路Tag | His路Tag,S路Tag | Thrombin,Enterokinas | 閵嗏偓 |
| pET-31b | T7 lac | Ampicillin | His路Tag | 閵嗏偓 | 閵嗏偓 | Ketosterol isomerase |
| pET-32a,b,c | T7 lac | Ampicillin | thioredoxin | His路Tag,S路Tag | 閵嗏偓 | 閵嗏偓 |
| pET-33b | T7 lac | Kanamycin | His路Tag | His路Tag,T7路Tag | Thrombin | Protein kinase PKC recognition site |
| pET-37b | T7 lac | Kanamycin | His路Tag | CBD,S路Tag | Thrombin,Enterokinas | secretory expression,cellulose composite domain |
| pET-39b | T7 lac | Kanamycin | His路Tag | DsvA路Tag,His路Tag,S路Tag | Thrombin | Express DsvA fusion protein |
| pET-40b | T7 lac | Kanamycin | His路Tag | DsvC路Tag,His路Tag,S路Tag | Thrombin,Enterokinas | Express DsvC fusion protein |
| pET-41a,b,c | T7 lac | Kanamycin | His路Tag | GST路Tag,His路Tag,S路Tag | Thrombin,Enterokinas | 閵嗏偓 |
| pET-42a,b,c | T7 lac | Kanamycin | His路Tag | GST路Tag,His路Tag,S路Tag | Thrombin,Factor Xa | 閵嗏偓 |
| pET-43.1a,b | T7 lac | Ampicillin | HSV路Tag,His路Tag | Nus路Tag,His路Tag,S路Tag | Enterokinas,Thrombin | 閵嗏偓 |
| pET-44a,b,c | T7 lac | Ampicillin | HSV路Tag,His路Tag | His路Tag,Nus路Tag,His路Tag,S路Tag | Enterokinas,Thrombin | 閵嗏偓 |
| pET-45b | T7 lac | Ampicillin | S路Tag | His路Tag | Enterokinas | 閵嗏偓 |
| pET-47b | T7 lac | Kanamycin | S路Tag | His路Tag | HRV 3C,Thrombin | 閵嗏偓 |
| pET-48b | T7 lac | Kanamycin | S路Tag,S路Tag | Trx路Tag,His路Tag | HRV 3C,Thrombin | 閵嗏偓 |
| pET-49b | T7 lac | Kanamycin | S路Tag,S路Tag | GST路Tag,His路Tag | HRV 3C,Thrombin | 閵嗏偓 |
| pET-50b | T7 lac | Kanamycin | S路Tag,S路Tag | His路Tag,Nus路Tag,His路Tag | HRV 3C,Thrombin | 閵嗏偓 |
| pET-51 | T7 lac | Ampicillin | His路Tag,His路Tag | Strep路Tag 閳?/td> | Enterokinas,Enterokinas | 閵嗏偓 |
| pET-52b | T7 lac | Ampicillin | His路Tag,His路Tag | Strep路Tag 閳?/td> | HRV 3C,Thrombin | 閵嗏偓 |
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