pET-12b Plasmid
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pET-12b Plasmid

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  • 2025年08月31日
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    • 文献和实验
    • 技术资料
    • 保存条件：

      负20摄氏度

    • 保质期：

      3个月

    • 英文名：

      pET-12b

    • 库存：

      50

    • 供应商：

      LSMBIO

    • 规格：

      2ug

    pET-12b

    Search name

    pET-12b,Plasmid pET-12b,pET-12b vector

     

    pET-12b Information

    Plasmid type: Escherichia coli expression vector

    Expression level: high

    Cloning methods: polyclonal sites, restrictive endonucleases

    Carrier size: 4673bp

    5'sequencing primers: T7/ lacI

    Sequence of 5'sequencing primers: 5'-TAATACGACTCACTATAGGG-3'

    Carrier label: ompT

    Carrier resistance: Ampicillin

    Cloned strain: DH5a

    Expression strain: BL21 series

    Remarks:

    PET-12a, B, C only read the frame (read code box) different, each other 1 bp.

    Product directory number: -

    Stability: stable expression

    Composition type: inducible type (IPTG)

    Virus / non virus: non virus

     

    pET-12b Description

    Feature NameStartEnd 
    tet (w/ gaps)861586
    T7_terminator507379
    T7_Terminal_primer447465
    T7_transl_en_RBS605589
    T7_promoter660642
    pBRrevBam_primer763744
    ROP22252416
    pGEX_3_primer24322410
    Ampicillin44653605
    AmpR_promoter45354507
    ORFStartEnd 
    ORF frame 27971384
    ORF frame 11391906
    ORF frame 39691586
    ORF frame 244653605
    Enzyme NameCut
    ClaI24
    HindIII29
    NheI229
    BamHI510
    SalI516
    NdeI579
    XbaI617
    BglII675
    EagI1249
    MscI1756
    PvuII2376
    PstI3923
    AatII4600
    EcoRI4671

    pET-12b-polyclonal-site

     

    pET-Vectors -protocol    
           
    Vector NamePromoterResistanceC-tagN-tagProtease cleavage siteApplication
    pET-3a,b,cT7Ampicillin閵嗏偓T7路Tag閵嗏偓Constituent expression
    pET-11a,b,c,dT7 lacAmpicillin閵嗏偓T7路Tag閵嗏偓閵嗏偓
    pET-12aT7 lacAmpicillin閵嗏偓ompT閵嗏偓閵嗏偓
    pET-14bT7 lacAmpicillin閵嗏偓His路TagThrombin閵嗏偓
    pET-15bT7 lacAmpicillin閵嗏偓His路TagThrombin閵嗏偓
    pET-16bT7 lacAmpicillin閵嗏偓His路TagFactor Xa閵嗏偓
    pET-17bT7 lacAmpicillin閵嗏偓T7路Tag閵嗏偓閵嗏偓
    pET-19bT7 lacAmpicillin閵嗏偓His路TagEnterokinas閵嗏偓
    pET-20bT7 lacAmpicillinHis路TagpelB signal peptide閵嗏偓secretory expression
    pET-21a,b,c,dT7 lacAmpicillinHis路TagT7路Tag閵嗏偓閵嗏偓
    pET-22bT7 lacAmpicillinHis路TagpelB signal peptide閵嗏偓secretory expression
    pET-23a,b,c,dT7AmpicillinHis路TagT7路Tag閵嗏偓Constituent expression
    pET-24a,b,c,dT7 lacKanamycinHis路TagT7路Tag閵嗏偓閵嗏偓
    pET-25bT7 lacAmpicillinHSV路Tag,His路TagpelB signal peptide閵嗏偓secretory expression
    pET-26bT7 lacKanamycinHis路TagpelB signal peptide閵嗏偓secretory expression
    pET-27bT7 lacKanamycinHSV路Tag,His路TagpelB signal peptide閵嗏偓secretory expression
    pET-28a,b,cT7 lacKanamycinHis路TagHis路Tag,T7路TagThrombin閵嗏偓
    pET-29aT7 lacKanamycinHis路TagS路TagThrombin閵嗏偓
    pET-30a,b,cT7 lacKanamycinHis路TagHis路Tag,S路TagThrombin,Enterokinas閵嗏偓
    pET-31bT7 lacAmpicillinHis路Tag閵嗏偓閵嗏偓Ketosterol isomerase
    pET-32a,b,cT7 lacAmpicillinthioredoxinHis路Tag,S路Tag閵嗏偓閵嗏偓
    pET-33bT7 lacKanamycinHis路TagHis路Tag,T7路TagThrombinProtein kinase PKC recognition site
    pET-37bT7 lacKanamycinHis路TagCBD,S路TagThrombin,Enterokinassecretory expression,cellulose composite domain
    pET-39bT7 lacKanamycinHis路TagDsvA路Tag,His路Tag,S路TagThrombinExpress DsvA fusion protein
    pET-40bT7 lacKanamycinHis路TagDsvC路Tag,His路Tag,S路TagThrombin,EnterokinasExpress DsvC fusion protein
    pET-41a,b,cT7 lacKanamycinHis路TagGST路Tag,His路Tag,S路TagThrombin,Enterokinas閵嗏偓
    pET-42a,b,cT7 lacKanamycinHis路TagGST路Tag,His路Tag,S路TagThrombin,Factor Xa閵嗏偓
    pET-43.1a,bT7 lacAmpicillinHSV路Tag,His路TagNus路Tag,His路Tag,S路TagEnterokinas,Thrombin閵嗏偓
    pET-44a,b,cT7 lacAmpicillinHSV路Tag,His路TagHis路Tag,Nus路Tag,His路Tag,S路TagEnterokinas,Thrombin閵嗏偓
    pET-45bT7 lacAmpicillinS路TagHis路TagEnterokinas閵嗏偓
    pET-47bT7 lacKanamycinS路TagHis路TagHRV 3C,Thrombin閵嗏偓
    pET-48bT7 lacKanamycinS路Tag,S路TagTrx路Tag,His路TagHRV 3C,Thrombin閵嗏偓
    pET-49bT7 lacKanamycinS路Tag,S路TagGST路Tag,His路TagHRV 3C,Thrombin閵嗏偓
    pET-50bT7 lacKanamycinS路Tag,S路TagHis路Tag,Nus路Tag,His路TagHRV 3C,Thrombin閵嗏偓
    pET-51T7 lacAmpicillinHis路Tag,His路TagStrep路Tag 閳?/td> Enterokinas,Enterokinas閵嗏偓
    pET-52bT7 lacAmpicillinHis路Tag,His路TagStrep路Tag 閳?/td> HRV 3C,Thrombin閵嗏偓
     

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    图标文献和实验
    该产品被引用文献
    Structural basis for the interaction of the chaperone Cbp3 with newly synthesized cytochrome b during mitochondrial respiratory chain assembly Mama Ndi1, Geoffrey Masuyer1, Hannah Dawitz1, Andreas Carlstr?m1, Mirco Michel1, Arne Elofsson2, Mikaela Rapp1, P?l Stenmark3 and Martin Ott3* First Published on Journal of biological chemistry September 19, 2019 doi: 10.1074/jbc.RA119.010483 jbc.RA119.010483.
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