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- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pET-12b
- 库存：
大量
- 供应商：
联迈生物
- 规格：
1ug
pET-12b
质粒名称: pET-12b
其他名称: pET12b
质粒大小（bp）: 4673
抗性: Ampicillin (氨苄青霉素)
质粒类型: 大肠杆菌表达载体
是否病毒: 非病毒
启动子: T7
稳转或瞬转: Unspecified
组成型或诱导性: Unspecified
表达水平:
蛋白标签:
5′ 测序引物及序列: T7：5′-TAATACGACTCACTATAGGG-3′
3’测序引物及序列：无
GenBank号:
其他属性: Hosts: E.coli. Related vectors: pBR322.
质粒图谱:
质粒序列:
| 1 | TTCTCATGTT | TGACAGCTTA | TCATCGATAA | GCTTTAATGC | GGTAGTTTAT | 50 |
| 51 | CACAGTTAAA | TTGCTAACGC | AGTCAGGCAC | CGTGTATGAA | ATCTAACAAT | 100 |
| 101 | GCGCTCATCG | TCATCCTCGG | CACCGTCACC | CTGGATGCTG | TAGGCATAGG | 150 |
| 151 | CTTGGTTATG | CCGGTACTGC | CGGGCCTCTT | GCGGGATATC | GTCCATTCCG | 200 |
| 201 | ACAGCATCGC | CAGTCACTAT | GGCGTGCTGC | TAGCGCTATA | TGCGTTGATG | 250 |
| 251 | CAATTTCTAT | GCGCACCCGT | TCTCGGAGCA | CTGTCCGACC | GCTTTGGCCG | 300 |
| 301 | CCGCCCAGTC | CTGCTCGCTT | CGCTACTTGG | AGCCACTATC | GACTACGCGA | 350 |
| 351 | TCATGGCGAC | CACACCCGTC | CTGTGGATAT | CCGGATATAG | TTCCTCCTTT | 400 |
| 401 | CAGCAAAAAA | CCCCTCAAGA | CCCGTTTAGA | GGCCCCAAGG | GGTTATGCTA | 450 |
| 451 | GTTATTGCTC | AGCGGTGGCA | GCAGCCAACT | CAGCTTCCTT | TCGGGCTTTG | 500 |
| 501 | TTAGCAGCCG | GATCCGTCGA | CGCAAAAGAG | CTGATCGCGA | TAGGGGTTGT | 550 |
| 551 | CAGGACTATT | CCTAGGAGTT | TCGCCCGCAT | ATGTATATCT | CCTTCTTAAA | 600 |
| 601 | GTTAAACAAA | ATTATTTCTA | GAGGGAAACC | GTTGTGGTCT | CCCTATAGTG | 650 |
| 651 | AGTCGTATTA | ATTTCGCGGG | ATCGAGATCT | CGATCCTCTA | CGCCGGACGC | 700 |
| 701 | ATCGTGGCCG | GCATCACCGG | CGCCACAGGT | GCGGTTGCTG | GCGCCTATAT | 750 |
| 751 | CGCCGACATC | ACCGATGGGG | AAGATCGGGC | TCGCCACTTC | GGGCTCATGA | 800 |
| 801 | GCGCTTGTTT | CGGCGTGGGT | ATGGTGGCAG | GCCCCGTGGC | CGGGGGACTG | 850 |
| 851 | TTGGGCGCCA | TCTCCTTGCA | TGCACCATTC | CTTGCGGCGG | CGGTGCTCAA | 900 |
| 901 | CGGCCTCAAC | CTACTACTGG | GCTGCTTCCT | AATGCAGGAG | TCGCATAAGG | 950 |
| 951 | GAGAGCGTCG | ATCGACCGAT | GCCCTTGAGA | GCCTTCAACC | CAGTCAGCTC | 1000 |
| 1001 | CTTCCGGTGG | GCGCGGGGCA | TGACTATCGT | CGCCGCACTT | ATGACTGTCT | 1050 |
| 1051 | TCTTTATCAT | GCAACTCGTA | GGACAGGTGC | CGGCAGCGCT | CTGGGTCATT | 1100 |
| 1101 | TTCGGCGAGG | ACCGCTTTCG | CTGGAGCGCG | ACGATGATCG | GCCTGTCGCT | 1150 |
| 1151 | TGCGGTATTC | GGAATCTTGC | ACGCCCTCGC | TCAAGCCTTC | GTCACTGGTC | 1200 |
| 1201 | CCGCCACCAA | ACGTTTCGGC | GAGAAGCAGG | CCATTATCGC | CGGCATGGCG | 1250 |
| 1251 | GCCGACGCGC | TGGGCTACGT | CTTGCTGGCG | TTCGCGACGC | GAGGCTGGAT | 1300 |
| 1301 | GGCCTTCCCC | ATTATGATTC | TTCTCGCTTC | CGGCGGCATC | GGGATGCCCG | 1350 |
| 1351 | CGTTGCAGGC | CATGCTGTCC | AGGCAGGTAG | ATGACGACCA | TCAGGGACAG | 1400 |
| 1401 | CTTCAAGGAT | CGCTCGCGGC | TCTTACCAGC | CTAACTTCGA | TCACTGGACC | 1450 |
| 1451 | GCTGATCGTC | ACGGCGATTT | ATGCCGCCTC | GGCGAGCACA | TGGAACGGGT | 1500 |
| 1501 | TGGCATGGAT | TGTAGGCGCC | GCCCTATACC | TTGTCTGCCT | CCCCGCGTTG | 1550 |
| 1551 | CGTCGCGGTG | CATGGAGCCG | GGCCACCTCG | ACCTGAATGG | AAGCCGGCGG | 1600 |
| 1601 | CACCTCGCTA | ACGGATTCAC | CACTCCAAGA | ATTGGAGCCA | ATCAATTCTT | 1650 |
| 1651 | GCGGAGAACT | GTGAATGCGC | AAACCAACCC | TTGGCAGAAC | ATATCCATCG | 1700 |
| 1701 | CGTCCGCCAT | CTCCAGCAGC | CGCACGCGGC | GCATCTCGGG | CAGCGTTGGG | 1750 |
| 1751 | TCCTGGCCAC | GGGTGCGCAT | GATCGTGCTC | CTGTCGTTGA | GGACCCGGCT | 1800 |
| 1801 | AGGCTGGCGG | GGTTGCCTTA | CTGGTTAGCA | GAATGAATCA | CCGATACGCG | 1850 |
| 1851 | AGCGAACGTG | AAGCGACTGC | TGCTGCAAAA | CGTCTGCGAC | CTGAGCAACA | 1900 |
| 1901 | ACATGAATGG | TCTTCGGTTT | CCGTGTTTCG | TAAAGTCTGG | AAACGCGGAA | 1950 |
| 1951 | GTCAGCGCCC | TGCACCATTA | TGTTCCGGAT | CTGCATCGCA | GGATGCTGCT | 2000 |
| 2001 | GGCTACCCTG | TGGAACACCT | ACATCTGTAT | TAACGAAGCG | CTGGCATTGA | 2050 |
| 2051 | CCCTGAGTGA | TTTTTCTCTG | GTCCCGCCGC | ATCCATACCG | CCAGTTGTTT | 2100 |
| 2101 | ACCCTCACAA | CGTTCCAGTA | ACCGGGCATG | TTCATCATCA | GTAACCCGTA | 2150 |
| 2151 | TCGTGAGCAT | CCTCTCTCGT | TTCATCGGTA | TCATTACCCC | CATGAACAGA | 2200 |
| 2201 | AATCCCCCTT | ACACGGAGGC | ATCAGTGACC | AAACAGGAAA | AAACCGCCCT | 2250 |
| 2251 | TAACATGGCC | CGCTTTATCA | GAAGCCAGAC | ATTAACGCTT | CTGGAGAAAC | 2300 |
| 2301 | TCAACGAGCT | GGACGCGGAT | GAACAGGCAG | ACATCTGTGA | ATCGCTTCAC | 2350 |
| 2351 | GACCACGCTG | ATGAGCTTTA | CCGCAGCTGC | CTCGCGCGTT | TCGGTGATGA | 2400 |
| 2401 | CGGTGAAAAC | CTCTGACACA | TGCAGCTCCC | GGAGACGGTC | ACAGCTTGTC | 2450 |
| 2451 | TGTAAGCGGA | TGCCGGGAGC | AGACAAGCCC | GTCAGGGCGC | GTCAGCGGGT | 2500 |
| 2501 | GTTGGCGGGT | GTCGGGGCGC | AGCCATGACC | CAGTCACGTA | GCGATAGCGG | 2550 |
| 2551 | AGTGTATACT | GGCTTAACTA | TGCGGCATCA | GAGCAGATTG | TACTGAGAGT | 2600 |
| 2601 | GCACCATATA | TGCGGTGTGA | AATACCGCAC | AGATGCGTAA | GGAGAAAATA | 2650 |
| 2651 | CCGCATCAGG | CGCTCTTCCG | CTTCCTCGCT | CACTGACTCG | CTGCGCTCGG | 2700 |
| 2701 | TCGTTCGGCT | GCGGCGAGCG | GTATCAGCTC | ACTCAAAGGC | GGTAATACGG | 2750 |
| 2751 | TTATCCACAG | AATCAGGGGA | TAACGCAGGA | AAGAACATGT | GAGCAAAAGG | 2800 |
| 2801 | CCAGCAAAAG | GCCAGGAACC | GTAAAAAGGC | CGCGTTGCTG | GCGTTTTTCC | 2850 |
| 2851 | ATAGGCTCCG | CCCCCCTGAC | GAGCATCACA | AAAATCGACG | CTCAAGTCAG | 2900 |
| 2901 | AGGTGGCGAA | ACCCGACAGG | ACTATAAAGA | TACCAGGCGT | TTCCCCCTGG | 2950 |
| 2951 | AAGCTCCCTC | GTGCGCTCTC | CTGTTCCGAC | CCTGCCGCTT | ACCGGATACC | 3000 |
| 3001 | TGTCCGCCTT | TCTCCCTTCG | GGAAGCGTGG | CGCTTTCTCA | TAGCTCACGC | 3050 |
| 3051 | TGTAGGTATC | TCAGTTCGGT | GTAGGTCGTT | CGCTCCAAGC | TGGGCTGTGT | 3100 |
| 3101 | GCACGAACCC | CCCGTTCAGC | CCGACCGCTG | CGCCTTATCC | GGTAACTATC | 3150 |
| 3151 | GTCTTGAGTC | CAACCCGGTA | AGACACGACT | TATCGCCACT | GGCAGCAGCC | 3200 |
| 3201 | ACTGGTAACA | GGATTAGCAG | AGCGAGGTAT | GTAGGCGGTG | CTACAGAGTT | 3250 |
| 3251 | CTTGAAGTGG | TGGCCTAACT | ACGGCTACAC | TAGAAGGACA | GTATTTGGTA | 3300 |
| 3301 | TCTGCGCTCT | GCTGAAGCCA | GTTACCTTCG | GAAAAAGAGT | TGGTAGCTCT | 3350 |
| 3351 | TGATCCGGCA | AACAAACCAC | CGCTGGTAGC | GGTGGTTTTT | TTGTTTGCAA | 3400 |
| 3401 | GCAGCAGATT | ACGCGCAGAA | AAAAAGGATC | TCAAGAAGAT | CCTTTGATCT | 3450 |
| 3451 | TTTCTACGGG | GTCTGACGCT | CAGTGGAACG | AAAACTCACG | TTAAGGGATT | 3500 |
| 3501 | TTGGTCATGA | GATTATCAAA | AAGGATCTTC | ACCTAGATCC | TTTTAAATTA | 3550 |
| 3551 | AAAATGAAGT | TTTAAATCAA | TCTAAAGTAT | ATATGAGTAA | ACTTGGTCTG | 3600 |
| 3601 | ACAGTTACCA | ATGCTTAATC | AGTGAGGCAC | CTATCTCAGC | GATCTGTCTA | 3650 |
| 3651 | TTTCGTTCAT | CCATAGTTGC | CTGACTCCCC | GTCGTGTAGA | TAACTACGAT | 3700 |
| 3701 | ACGGGAGGGC | TTACCATCTG | GCCCCAGTGC | TGCAATGATA | CCGCGAGACC | 3750 |
| 3751 | CACGCTCACC | GGCTCCAGAT | TTATCAGCAA | TAAACCAGCC | AGCCGGAAGG | 3800 |
| 3801 | GCCGAGCGCA | GAAGTGGTCC | TGCAACTTTA | TCCGCCTCCA | TCCAGTCTAT | 3850 |
| 3851 | TAATTGTTGC | CGGGAAGCTA | GAGTAAGTAG | TTCGCCAGTT | AATAGTTTGC | 3900 |
| 3901 | GCAACGTTGT | TGCCATTGCT | GCAGGCATCG | TGGTGTCACG | CTCGTCGTTT | 3950 |
| 3951 | GGTATGGCTT | CATTCAGCTC | CGGTTCCCAA | CGATCAAGGC | GAGTTACATG | 4000 |
| 4001 | ATCCCCCATG | TTGTGCAAAA | AAGCGGTTAG | CTCCTTCGGT | CCTCCGATCG | 4050 |
| 4051 | TTGTCAGAAG | TAAGTTGGCC | GCAGTGTTAT | CACTCATGGT | TATGGCAGCA | 4100 |
| 4101 | CTGCATAATT | CTCTTACTGT | CATGCCATCC | GTAAGATGCT | TTTCTGTGAC | 4150 |
| 4151 | TGGTGAGTAC | TCAACCAAGT | CATTCTGAGA | ATAGTGTATG | CGGCGACCGA | 4200 |
| 4201 | GTTGCTCTTG | CCCGGCGTCA | ACACGGGATA | ATACCGCGCC | ACATAGCAGA | 4250 |
| 4251 | ACTTTAAAAG | TGCTCATCAT | TGGAAAACGT | TCTTCGGGGC | GAAAACTCTC | 4300 |
| 4301 | AAGGATCTTA | CCGCTGTTGA | GATCCAGTTC | GATGTAACCC | ACTCGTGCAC | 4350 |
| 4351 | CCAACTGATC | TTCAGCATCT | TTTACTTTCA | CCAGCGTTTC | TGGGTGAGCA | 4400 |
| 4401 | AAAACAGGAA | GGCAAAATGC | CGCAAAAAAG | GGAATAAGGG | CGACACGGAA | 4450 |
| 4451 | ATGTTGAATA | CTCATACTCT | TCCTTTTTCA | ATATTATTGA | AGCATTTATC | 4500 |
| 4501 | AGGGTTATTG | TCTCATGAGC | GGATACATAT | TTGAATGTAT | TTAGAAAAAT | 4550 |
| 4551 | AAACAAATAG | GGGTTCCGCG | CACATTTCCC | CGAAAAGTGC | CACCTGACGT | 4600 |
| 4601 | CTAAGAAACC | ATTATTATCA | TGACATTAAC | CTATAAAAAT | AGGCGTATCA | 4650 |
| 4651 | CGAGGCCCTT | TCGTCTTCAA | GAA | 4673 |
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pET-12b
¥1 - 1880
