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- LSM Bio
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- PVT6332
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pdCas9- Bacteria
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pdCas9-Bacteria
Search name
pdCas9-Bacteria,Plasmid pdCas9-Bacteria,pdCas9-Bacteria vector
pdCas9-Bacteria Information
Bacterial Resistance:Ampicillin
Growth Strain:
Use:CRISPR-CAS41-sgRNA plasmid
pdCas9-Bacteria Sequence
gacgtcttaagacccactttcacatttaagttgtttttctaatccgcatatgatcaattcaaggccgaat
aagaaggctggctctgcaccttggtgatcaaataattcgatagcttgtcgtaataatggcggcatactat
cagtagtaggtgtttccctttcttctttagcgacttgatgctcttgatcttccaatacgcaacctaaagt
aaaatgccccacagcgctgagtgcatataatgcattctctagtgaaaaaccttgttggcataaaaaggct
aattgattttcgagagtttcatactgtttttctgtaggccgtgtacctaaatgtacttttgctccatcgc
gatgacttagtaaagcacatctaaaacttttagcgttattacgtaaaaaatcttgccagctttccccttc
taaagggcaaaagtgagtatggtgcctatctaacatctcaatggctaaggcgtcgagcaaagcccgctta
ttttttacatgccaatacaatgtaggctgctctacacctagcttctgggcgagtttacgggttgttaaac
cttcgattccgacctcattaagcagctctaatgcgctgttaatcactttacttttatctaatctagacat
cattaattcctaatttttgttgacactctatcgttgatagagttattttaccactccctatcagtgatag
agaaaagaattcaaaaGATCTAAAGAGGAGAAAGGATCTATGGATAAGAAATACTCAATAGGCTTAGCTA
TCGGCACAAATAGCGTCGGATGGGCGGTGATCACTGATGAATATAAGGTTCCGTCTAAAAAGTTCAAGGT
TCTGGGAAATACAGACCGCCACAGTATCAAAAAAAATCTTATAGGGGCTCTTTTATTTGACAGTGGAGAG
ACAGCGGAAGCGACTCGTCTCAAACGGACAGCTCGTAGAAGGTATACACGTCGGAAGAATCGTATTTGTT
ATCTACAGGAGATTTTTTCAAATGAGATGGCGAAAGTAGATGATAGTTTCTTTCATCGACTTGAAGAGTC
TTTTTTGGTGGAAGAAGACAAGAAGCATGAACGTCATCCTATTTTTGGAAATATAGTAGATGAAGTTGCT
TATCATGAGAAATATCCAACTATCTATCATCTGCGAAAAAAATTGGTAGATTCTACTGATAAAGCGGATT
TGCGCTTAATCTATTTGGCCTTAGCGCATATGATTAAGTTTCGTGGTCATTTTTTGATTGAGGGAGATTT
AAATCCTGATAATAGTGATGTGGACAAACTATTTATCCAGTTGGTACAAACCTACAATCAATTATTTGAA
GAAAACCCTATTAACGCAAGTGGAGTAGATGCTAAAGCGATTCTTTCTGCACGATTGAGTAAATCAAGAC
GATTAGAAAATCTCATTGCTCAGCTCCCCGGTGAGAAGAAAAATGGCTTATTTGGGAATCTCATTGCTTT
GTCATTGGGTTTGACCCCTAATTTTAAATCAAATTTTGATTTGGCAGAAGATGCTAAATTACAGCTTTCA
AAAGATACTTACGATGATGATTTAGATAATTTATTGGCGCAAATTGGAGATCAATATGCTGATTTGTTTT
TGGCAGCTAAGAATTTATCAGATGCTATTTTACTTTCAGATATCCTAAGAGTAAATACTGAAATAACTAA
GGCTCCCCTATCAGCTTCAATGATTAAACGCTACGATGAACATCATCAAGACTTGACTCTTTTAAAAGCT
TTAGTTCGACAACAACTTCCAGAAAAGTATAAAGAAATCTTTTTTGATCAATCAAAAAACGGATATGCAG
GTTATATTGATGGGGGAGCTAGCCAAGAAGAATTTTATAAATTTATCAAACCAATTTTAGAAAAAATGGA
TGGTACTGAGGAATTATTGGTGAAACTAAATCGTGAAGATTTGCTGCGCAAGCAACGGACCTTTGACAAC
GGCTCTATTCCCCATCAAATTCACTTGGGTGAGCTGCATGCTATTTTGAGAAGACAAGAAGACTTTTATC
CATTTTTAAAAGACAATCGTGAGAAGATTGAAAAAATCTTGACTTTTCGAATTCCTTATTATGTTGGTCC
ATTGGCGCGTGGCAATAGTCGTTTTGCATGGATGACTCGGAAGTCTGAAGAAACAATTACCCCATGGAAT
TTTGAAGAAGTTGTCGATAAAGGTGCTTCAGCTCAATCATTTATTGAACGCATGACAAACTTTGATAAAA
ATCTTCCAAATGAAAAAGTACTACCAAAACATAGTTTGCTTTATGAGTATTTTACGGTTTATAACGAATT
GACAAAGGTCAAATATGTTACTGAAGGAATGCGAAAACCAGCATTTCTTTCAGGTGAACAGAAGAAAGCC
ATTGTTGATTTACTCTTCAAAACAAATCGAAAAGTAACCGTTAAGCAATTAAAAGAAGATTATTTCAAAA
AAATAGAATGTTTTGATAGTGTTGAAATTTCAGGAGTTGAAGATAGATTTAATGCTTCATTAGGTACCTA
CCATGATTTGCTAAAAATTATTAAAGATAAAGATTTTTTGGATAATGAAGAAAATGAAGATATCTTAGAG
GATATTGTTTTAACATTGACCTTATTTGAAGATAGGGAGATGATTGAGGAAAGACTTAAAACATATGCTC
ACCTCTTTGATGATAAGGTGATGAAACAGCTTAAACGTCGCCGTTATACTGGTTGGGGACGTTTGTCTCG
AAAATTGATTAATGGTATTAGGGATAAGCAATCTGGCAAAACAATATTAGATTTTTTGAAATCAGATGGT
TTTGCCAATCGCAATTTTATGCAGCTGATCCATGATGATAGTTTGACATTTAAAGAAGACATTCAAAAAG
CACAAGTGTCTGGACAAGGCGATAGTTTACATGAACATATTGCAAATTTAGCTGGTAGCCCTGCTATTAA
AAAAGGTATTTTACAGACTGTAAAAGTTGTTGATGAATTGGTCAAAGTAATGGGGCGGCATAAGCCAGAA
AATATCGTTATTGAAATGGCACGTGAAAATCAGACAACTCAAAAGGGCCAGAAAAATTCGCGAGAGCGTA
TGAAACGAATCGAAGAAGGTATCAAAGAATTAGGAAGTCAGATTCTTAAAGAGCATCCTGTTGAAAATAC
TCAATTGCAAAATGAAAAGCTCTATCTCTATTATCTCCAAAATGGAAGAGACATGTATGTGGACCAAGAA
TTAGATATTAATCGTTTAAGTGATTATGATGTCGATGCCATTGTTCCACAAAGTTTCCTTAAAGACGATT
CAATAGACAATAAGGTCTTAACGCGTTCTGATAAAAATCGTGGTAAATCGGATAACGTTCCAAGTGAAGA
AGTAGTCAAAAAGATGAAAAACTATTGGAGACAACTTCTAAACGCCAAGTTAATCACTCAACGTAAGTTT
GATAATTTAACGAAAGCTGAACGTGGAGGTTTGAGTGAACTTGATAAAGCTGGTTTTATCAAACGCCAAT
TGGTTGAAACTCGCCAAATCACTAAGCATGTGGCACAAATTTTGGATAGTCGCATGAATACTAAATACGA
TGAAAATGATAAACTTATTCGAGAGGTTAAAGTGATTACCTTAAAATCTAAATTAGTTTCTGACTTCCGA
AAAGATTTCCAATTCTATAAAGTACGTGAGATTAACAATTACCATCATGCCCATGATGCGTATCTAAATG
CCGTCGTTGGAACTGCTTTGATTAAGAAATATCCAAAACTTGAATCGGAGTTTGTCTATGGTGATTATAA
AGTTTATGATGTTCGTAAAATGATTGCTAAGTCTGAGCAAGAAATAGGCAAAGCAACCGCAAAATATTTC
TTTTACTCTAATATCATGAACTTCTTCAAAACAGAAATTACACTTGCAAATGGAGAGATTCGCAAACGCC
CTCTAATCGAAACTAATGGGGAAACTGGAGAAATTGTCTGGGATAAAGGGCGAGATTTTGCCACAGTGCG
CAAAGTATTGTCCATGCCCCAAGTCAATATTGTCAAGAAAACAGAAGTACAGACAGGCGGATTCTCCAAG
GAGTCAATTTTACCAAAAAGAAATTCGGACAAGCTTATTGCTCGTAAAAAAGACTGGGATCCAAAAAAAT
ATGGTGGTTTTGATAGTCCAACGGTAGCTTATTCAGTCCTAGTGGTTGCTAAGGTGGAAAAAGGGAAATC
GAAGAAGTTAAAATCCGTTAAAGAGTTACTAGGGATCACAATTATGGAAAGAAGTTCCTTTGAAAAAAAT
CCGATTGACTTTTTAGAAGCTAAAGGATATAAGGAAGTTAAAAAAGACTTAATCATTAAACTACCTAAAT
ATAGTCTTTTTGAGTTAGAAAACGGTCGTAAACGGATGCTGGCTAGTGCCGGAGAATTACAAAAAGGAAA
TGAGCTGGCTCTGCCAAGCAAATATGTGAATTTTTTATATTTAGCTAGTCATTATGAAAAGTTGAAGGGT
AGTCCAGAAGATAACGAACAAAAACAATTGTTTGTGGAGCAGCATAAGCATTATTTAGATGAGATTATTG
AGCAAATCAGTGAATTTTCTAAGCGTGTTATTTTAGCAGATGCCAATTTAGATAAAGTTCTTAGTGCATA
TAACAAACATAGAGACAAACCAATACGTGAACAAGCAGAAAATATTATTCATTTATTTACGTTGACGAAT
CTTGGAGCTCCCGCTGCTTTTAAATATTTTGATACAACAATTGATCGTAAACGATATACGTCTACAAAAG
AAGTTTTAGATGCCACTCTTATCCATCAATCCATCACTGGTCTTTATGAAACACGCATTGATTTGAGTCA
GCTAGGAGGTGACTAACtcgagtaaggatctccaggcatcaaataaaacgaaaggctcagtcgaaagact
gggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcggg
tgggcctttctgcgtttatacctagggatatattccgcttcctcgctcactgactcgctacgctcggtcg
ttcgactgcggcgagcggaaatggcttacgaacggggcggagatttcctggaagatgccaggaagatact
taacagggaagtgagagggccgcggcaaagccgtttttccataggctccgcccccctgacaagcatcacg
aaatctgacgctcaaatcagtggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg
cggctccctcgtgcgctctcctgttcctgcctttcggtttaccggtgtcattccgctgttatggccgcgt
ttgtctcattccacgcctgacactcagttccgggtaggcagttcgctccaagctggactgtatgcacgaa
ccccccgttcagtccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggaaagacatg
caaaagcaccactggcagcagccactggtaattgatttagaggagttagtcttgaagtcatgcgccggtt
aaggctaaactgaaaggacaagttttggtgactgcgctcctccaagccagttacctcggttcaaagagtt
ggtagctcagagaaccttcgaaaaaccgccctgcaaggcggttttttcgttttcagagcaagagattacg
cgcagaccaaaacgatctcaagaagatcatcttattaatcagataaaatatttctagatttcagtgcaat
ttatctcttcaaatgtagcacctgaagtcagccccatacgatataagttgttactagtgcttggattctc
accaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagttctgaggtcattact
ggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgcagtac
tgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgcca
gcggcatcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtc
catattggccacgtttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattc
tcaataaaccctttagggaaataggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgta
gaaactgccggaaatcgtcgtggtattcactccagagcgatgaaaacgtttcagtttgctcatggaaaac
ggtgtaacaagggtgaacactatcccatatcaccagctcaccgtctttcattgccatacgaaattccgga
tgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaacttgtgcttatttttctttacgg
tctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaactgactgaaatgc
ctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctccatt
ttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattat
ggtgaaagttggaacctcttacgtgccgatcaacgtctcattttcgccagatatc
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文献和实验, and then washing with electroporation buffer [0.9M sucrose,3mM MgCl2(pH 7.4)] one time, resuspended in electroporation buffer (150uL) d.Add 1.5uL DNA in TE buffer(0.1-1ug) into 50 uL bacteria, mix, 5min on ice. Then add into 1mm gap cuvettee, electroporate
-100ul酚氯仿，剧烈振荡5-10分种后，使用氯仿抽取去除酚和蛋白，然后按照其他步骤沉淀就可以得到你的质粒 ，DNA的提取也可以使用这种方法。 2.反复冻融破壁：培养好的1ml酵母细胞在STE溶液中洗涤两次后，加入200ul缓冲液[2% Triton X-100, 1% SDS, 100 mM NaCl, 10 mM Tris-HCl (pH 8.0), 1 mM EDTA (pH 8.0)]，使用液氮和96-98度沸水反复冻融3-5次，然后使用酚氯仿抽提蛋白!其余步骤参考其他文献
Nature 子刊：超声波调控肿瘤内细菌的基因表达，助力肿瘤免疫治疗
8 月 2 日，中国科学院深圳先进技术研究院合成所严飞研究员团队与南华大学附属第一医院陈智毅教授团队合作的最新成果以 Spatiotemporal control of engineered bacteria to express interferon-γ by focused ultrasound for tumor immunotherapy 为题在线发表于国际知名期刊 Nature communications。 在该工作中，研究团队开发了一种基于聚焦超声调控细菌基因表达免疫
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