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- PVTB00056-2a
- 2025年07月09日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
负20摄氏度
- 保质期：
3个月
- 英文名：
pFastBac 1-signal-CD43-His6
- 库存：
100
- 供应商：
LSMBIO
- 规格：
2ug
pFastBac 1-signal-CD43-His6 Plasmid
Catalog No. PVTB00056-2a
Vector Backbone pFastBac?1
Backbone manufacturer Invitrogen
Vector type Insect Expression
Backbone size w/o insert 4775bp
Cloning site 5 Xba I
Site destroyed during cloning NO
Cloning site 3 Hind III
Bacterial resistance Ampicillin
Growth strain DH5alpha
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Gentamicin
Background Insect Expression vector of Homo sapiens sialophorin
Gene Information
Alternative Gene Name SPN; LSN; CD43; GALGP; GPL115
Insert Gene name CD43
Insert size 1203bp
Accessions NM_001030288.2
Tag His(C-term)
Species Homo sapiens (human)
Gene ID 6693
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文献和实验Generation of Plant Protein Microarrays and Investigation of Antigen-Antibody Interactions
His-tagged plant proteins. After expression and purification of these recombinant proteins in high throughput, protein microarrays are generated utilizing a contact printer. For the detection of the recombinant proteins on the microarrays, an anti-RGS-His6
Mammalian Membrane Protein Expression in Baculovirus-Infected Insect Cells
, pFastBac Dual. The method is detailed to allow the expression of such proteins and membrane protein in general.
Identification and Analysis of Multiprotein Complexes Through Chemical Crosslinking
for two crosslinking methods considered to be particularly useful for the analysis of large multiprotein complexes are provided: His6?mediated crosslinking and photoinducible label transfer crosslinking. Keywords: Photoinducible crosslinking; label
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