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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
负20摄氏度
- 保质期:
3个月
- 英文名:
pFastBac 1-signal peptide-EGFR(1-621aa)-His6
- 库存:
100
- 供应商:
LSMBIO
- 规格:
2ug
pFastBac 1-signal peptide-EGFR(1-621aa)-His6 Plasmid
Catalog No. PVTB00063-2a
Vector Backbone pFastBac?1
Backbone manufacturer Invitrogen
Vector type Insect Expression
Backbone size w/o insert 4775bp
Cloning site 5 Xba I
Site destroyed during cloning NO
Cloning site 3 Hind III
Bacterial resistance Ampicillin
Growth strain DH5alpha
Growth temperature 37 Centigrade
High or low copy High Copy
Selectable markers Gentamicin
Background Insect Expression vector of Homo sapiens epidermal growth factor receptor (1-621aa)
Gene Information
Alternative Gene Name ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Insert Gene name EGFR
Insert size 1866bp
Accessions NG_007726.1
Tag His(C-term)
Species Homo sapiens (human)
Gene ID 1956
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文献和实验信号肽 signal peptide 某种分泌蛋白质及细胞膜蛋白质等,以前体物质多肽的形式合成,其 N末端含有作为通过膜时之信号的氨基酸序列,这种氨基酸序列称信号肽或信号序列( signal sequence)。由约 15— 25个氨基酸所组成,在 N末端附近除有碱性氨基酸外,还主要含疏水性氨基酸,特别是在其中部没有带电荷的氨基酸。在合成过程中前体物质多肽靠此序列与膜结合,接着形成膜结合型多核糖体,多肽链与其合成相平行地通过膜,并在通过膜时,由在存在膜上的肽酶将信号肽切断而除掉
In Vitro and In Vivo Approaches to Studying the Bacterial Signal Peptide Processing
across the cytoplasmic membrane, the signal peptide is proteolytically removed by signal peptide cleavage. Here, in this chapter, we describe methods to study signal peptide processing in bacteria, including purification of signal peptidase and its substrates
Prediction of Transmembrane Topology and Signal Peptide Given a Proteins Amino Acid Sequence
Here, we describe transmembrane topology and signal peptide predictors and highlight their advantages and shortcomings. We also discuss the relation between these two types of prediction.
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