- ￥980
- LSM Bio
- CHC00059
- 2025年07月14日
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￥980
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
负80摄氏度
- 保质期：
6month
- 英文名：
X33 chemically Yeast Express Competent Cells
- 库存：
货源充足
- 供应商：
LSM Bio
- 规格：
0.1ml*10
X33 chemically Competent Cells
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文献和实验a chilled, sterile pipette tip to transfer the competent cells to chilled, sterile 17 x 100-mm polypropylene tubes. Store the cells on ice. Glass tubes should not be used since they lower the efficiency of transformation by ~10-fold
J-6B centrifuge)for 10 min.at 4℃.4度2500g离心10分钟。5、Resuspend cells gently in 80 ml of ice cold "TB".小心用80ml预冷TB重悬细胞。6、On ice for 10 minutes.(30min)菌液置冰上10分钟。7、Spin at 2500 x g (5000 rpm in a Sorvall GSA,5500 rpm in a Sorvall SS-34,or 3000 rpm
! pH 6.7 - 7.0 Note: Competent Cell s are fragile (cell wall is thought to be weakened),therefore treat the cells gently when preparing these cells.Do not vortex or pippette up and down to resuspend the cells.Do not spin
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