P4HA1| Human Prolyl 4-hydroxyl

Introduction of human 14-3-3 proteins

regulation.We report here the crystal structure of the human T-cell 14-3-3 isoform (tau)dimer at 2.6 A resolution.Each monomer (Mr 28K)is composed of an unusual arrangement of nine antiparallel alpha-helices organized as two structural domains.The dimer

Recombinant Collagen Trimers from Insect Cells and Yeast

have been unsuccessful, but active recombinant human prolyl 4-hydroxylase has been produced in insect cells and yeast by coexpression of its α- and β-subunits (7 ,7 ). We have recently shown that recombinant human type III collagen with a stable triple helix

蛋白质组学指南

complex was not known. Analysis by 2-D phosphoprotein mapping identified two distinct proteins that were greatly increased in phosphate content in reg1  mutants. Mixed-peptide sequencing identified these proteins as hexokinase II (Hxk2p) and the E1 subunit