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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.31-20NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human CD11d/Integrin alpha D
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1NG/ML
- 规格:
96T
CD11d/Integrin alpha D ELISA KIT|Human
Catalog EF008490
Packing 48T/96T
Reactivity Human
Detect Range 0.156-10ng/ml
CD11d/Integrin alpha D ELISA KIT Sensitivity< 0.094ng/ml
Application CD11d/Integrin alpha D ELISA KIT|Human is for detecting the concentration of Human CD11d/Integrin alpha D in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of CD11d/Integrin alpha D ELISA KIT|Human
The microtiter plate provided in CD11d/Integrin alpha D ELISA KIT|Human has been pre-coated with an Human CD11d/Integrin alpha D antibody specific to Human CD11d/Integrin alpha D. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human CD11d/Integrin alpha Dantibody preparation specific for Human CD11d/Integrin alpha D then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human CD11d/Integrin alpha D, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human CD11d/Integrin alpha Din the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that CD11d/Integrin alpha D ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Assay (Invitrogen) as suggested by Illumina. Illumina Bead Array MicroRNA Detection Starting with 500 ng/sample of total RNA, mature microRNAs were amplified with the Illumina human v1 MicroRNA expression profiling kit containing primers for 743
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
9. GlutaMAX™-I Supplement 10. Basic FGF, Recombinant Human 11. β-Mercaptoethanol, 1000X 12. Penicillin-Streptomycin, Liquid 13. Attachment Factor 14. TrypLE™ Select Cell Dissociation Reagent or 0.05% Trypsin/EDTA
Methods for the Detection of D-Amino-Acid Oxidase
al. (5 ) using bovine serum albumin as a standard. It was also determined using a Protein Assay Kit (Bio-Rad, Hercules, CA). D-Amino-acid oxidase activity is finally expressed as the amount of D-alanine oxidized per min per milligram of protein. Hog
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