- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-48185h
- 2025年09月10日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human E3 ubiquitin-protein ligase Mdm2,MDM2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human E3 ubiquitin- protein ligase Mdm2, MDM2 ELISA KIT
Product Name:Human E3 ubiquitin- protein ligase Mdm2, MDM2 ELISA KIT
Packing:96T
Catalog No.:ELI-48185h
Gene Name:MDM2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:E3 ubiquitin-protein ligase Mdm2(MDM2
Alternative Name:MDM2,E3 ubiquitin-protein ligase Mdm2(MDM2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human E3 ubiquitin- protein ligase Mdm2, MDM2 ELISA KIT allows for the in vitro quantitative determination of E3 ubiquitin-protein ligase Mdm2(MDM2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human E3 ubiquitin- protein ligase Mdm2, MDM2 ELISA KIT has been pre-coated with an E3 ubiquitin-protein ligase Mdm2(MDM2 antibody specific to E3 ubiquitin-protein ligase Mdm2(MDM2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E3 ubiquitin-protein ligase Mdm2(MDM2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin-protein ligase Mdm2(MDM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of E3 ubiquitin-protein ligase Mdm2(MDM2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
ubiquitin protein ligase activity to wild-type (but not mutant) Parkin proteins. Although the individual components of the proposed Parkin ubiquitin ligase complex in the normal human brain remain to be identified and the E3 ligase effect of Parkin function
Studies of the Ubiquitin Proteasome System
Protocol 10: Detection of E3 Activity in Immunoprecipitated Protein Alternate Protocol 5: Detection of Ubiquitin Modification of Substrates After GST Pull‐Down Detection of Ubiquitylation in Vivo Basic Protocol 11: Lysis and Immunoprecipitation
内蛋白降解的最普通机制，泛素化的蛋白质被蛋白酶体识别而降解。癌基因蛋白Mdm2作为泛素连接酶(E3 ligase)，可直接与P53蛋白结合，促进P53蛋白的泛素化，在P53的泛素化(ubiquitylation)和蛋白质降解中起关键作用。Mdm2亦可被p53激活，因而Mdm2是夕53基因的负反馈调节因子(neganve feedback regulator)。 DNA损伤及其他方面的细胞功能异常主要是通过阻止P53蛋白与Mdm2结合而激活P53蛋白。DNA损伤引起多种蛋白激酶激活，如DNA―PK
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