- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- EF007318
- 2025年07月12日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human HAV-IgM
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
HAV-IgM ELISA KIT|Human
Catalog EF007318
Packing 48T/96T
Reactivity Human
Detect Range Qualitative
HAV-IgM ELISA KIT Sensitivity< Qualitative
Application HAV-IgM ELISA KIT|Human is for detecting the concentration of Human HAV-IgM in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of HAV-IgM ELISA KIT|Human
The microtiter plate provided in HAV-IgM ELISA KIT|Human has been pre-coated with an Human HAV-IgM antibody specific to Human HAV-IgM. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human HAV-IgMantibody preparation specific for Human HAV-IgM then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human HAV-IgM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human HAV-IgMin the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that HAV-IgM ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Warnings And Limitations This product is for research use only. Not intended for any animal or human therapeutic or diagnostic use unless otherwise stated. Follow appropriate laboratory guidelines. This product contains 0.02% sodium azide
可作夹心法的两个以上的位点，因此不能用双抗体夹心法进行测定，可以采用竞争法模式。其原理是标本中的抗原和一定量的酶标抗原竞争与固相抗体结合。标本中抗原量含量愈多，结合在固相上的酶标抗原愈少，最后的显色也愈浅。小分子激素、药物等ELISA测定多用此法。 6.捕获包被法测抗体 IgM抗体的检测用于传染病的早期诊断中。间接法ELISA一般仅适用于检测总抗体或IgG抗体。如用抗原包被的间接法直接测定IgM抗体，因标本中一般同时存在较高浓度的IgG抗体，后者将竞争结合固相抗原而使一部份IgM抗体不能结合
人莱姆病IgM(Lyme-IgM)酶联免疫分析（ELISA）
人莱姆病 IgM(Lyme-IgM) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于检测人血清，血浆及相关液体样本中莱姆病IgM(Lyme-IgM) 水平。 实验原理： 本试剂盒采用双抗原夹心酶联免疫法（ ELISA ）测定标本中人莱姆病 IgM(Lyme-IgM) 。用纯化的抗原包被微孔板，制成固相抗原，可与样品中莱姆病 IgM(Lyme-IgM) 相结合 ，经洗
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