Target Protein Name:2-5A-dependent ribonuclease(RNASEL
Alternative Name:RNASEL,2-5A-dependent ribonuclease(RNASEL Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human 2- 5A- dependent ribonuclease, RNASEL ELISA KIT allows for the in vitro quantitative determination of 2-5A-dependent ribonuclease(RNASEL concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human 2- 5A- dependent ribonuclease, RNASEL ELISA KIT has been pre-coated with an 2-5A-dependent ribonuclease(RNASEL antibody specific to 2-5A-dependent ribonuclease(RNASEL .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 2-5A-dependent ribonuclease(RNASEL and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain 2-5A-dependent ribonuclease(RNASEL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of 2-5A-dependent ribonuclease(RNASEL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!