Application IL-8 ELISA KIT|Human is for detecting the concentration of Human IL-8 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of IL-8 ELISA KIT|Human
The microtiter plate provided in IL-8 ELISA KIT|Human has been pre-coated with an Human IL-8 antibody specific to Human IL-8. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human IL-8antibody preparation specific for Human IL-8 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human IL-8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human IL-8in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that IL-8 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!