Target Protein Name:UBX domain-containing protein 11(UBXN11
Alternative Name:UBXN11,UBX domain-containing protein 11(UBXN11 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human UBX domain- containing protein 11, UBXN11 ELISA KIT allows for the in vitro quantitative determination of UBX domain-containing protein 11(UBXN11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human UBX domain- containing protein 11, UBXN11 ELISA KIT has been pre-coated with an UBX domain-containing protein 11(UBXN11 antibody specific to UBX domain-containing protein 11(UBXN11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for UBX domain-containing protein 11(UBXN11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UBX domain-containing protein 11(UBXN11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of UBX domain-containing protein 11(UBXN11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!