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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human UBX domain-containing protein 11,UBXN11
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
Human UBX domain- containing protein 11, UBXN11 ELISA KIT
Product Name:Human UBX domain- containing protein 11, UBXN11 ELISA KIT
Packing:96T
Catalog No.:ELI-51338h
Gene Name:UBXN11
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:UBX domain-containing protein 11(UBXN11
Alternative Name:UBXN11,UBX domain-containing protein 11(UBXN11
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human UBX domain- containing protein 11, UBXN11 ELISA KIT allows for the in vitro quantitative determination of UBX domain-containing protein 11(UBXN11 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human UBX domain- containing protein 11, UBXN11 ELISA KIT has been pre-coated with an UBX domain-containing protein 11(UBXN11 antibody specific to UBX domain-containing protein 11(UBXN11 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for UBX domain-containing protein 11(UBXN11 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain UBX domain-containing protein 11(UBXN11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of UBX domain-containing protein 11(UBXN11 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Mutational Analysis of the Human Protein C Gene
The single gene for protein C is located at position q13–q14 on chromosome 2 (1 ). Two groups have described human genomic clones of protein C isolated from phage l charon libraries using cDNA for human protein C as hybridization probes
Protein Carbonyl Measurement by ELISA
) (2,3). Protein-bound DNP can also be measured with increased sensitivity by enzyme-linked immunosorbent assay (ELISA) (4,5). Samples containing protein are reacted with DNP, then the protein is nonspecifically adsorbed to an ELISA plate. Unconjugated DNP
Lambda gt11 cDNA libraries are routinely used for gene isolation with antibody and oligomer probes (1 –3 ). Briefly, a recombinant DNA library from different types of cells is constructed in a λgt 11 expression vector. The foreign peptides
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