- ￥2890
- LSM Bio
- 0.31-20NG/ML
- 0.1NG/ML
- 进口
- ELI-52352h
- 2025年07月11日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human E3 ubiquitin-protein ligase SMURF1,SMURF1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1NG/ML
- 规格：
96T
Human E3 ubiquitin- protein ligase SMURF1, SMURF1 ELISA KIT
Product Name:Human E3 ubiquitin- protein ligase SMURF1, SMURF1 ELISA KIT
Packing:96T
Catalog No.:ELI-52352h
Gene Name:SMURF1
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:E3 ubiquitin-protein ligase SMURF1(SMURF1
Alternative Name:SMURF1,E3 ubiquitin-protein ligase SMURF1(SMURF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human E3 ubiquitin- protein ligase SMURF1, SMURF1 ELISA KIT allows for the in vitro quantitative determination of E3 ubiquitin-protein ligase SMURF1(SMURF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human E3 ubiquitin- protein ligase SMURF1, SMURF1 ELISA KIT has been pre-coated with an E3 ubiquitin-protein ligase SMURF1(SMURF1 antibody specific to E3 ubiquitin-protein ligase SMURF1(SMURF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for E3 ubiquitin-protein ligase SMURF1(SMURF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain E3 ubiquitin-protein ligase SMURF1(SMURF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of E3 ubiquitin-protein ligase SMURF1(SMURF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Parkinsons Disease: Assays for the Ubiquitin Ligase Activity of Neural Parkin
ubiquitin protein ligase activity to wild-type (but not mutant) Parkin proteins. Although the individual components of the proposed Parkin ubiquitin ligase complex in the normal human brain remain to be identified and the E3 ligase effect of Parkin function
Studies of the Ubiquitin Proteasome System
Protocol 10: Detection of E3 Activity in Immunoprecipitated Protein Alternate Protocol 5: Detection of Ubiquitin Modification of Substrates After GST Pull‐Down Detection of Ubiquitylation in Vivo Basic Protocol 11: Lysis and Immunoprecipitation
In Vitro Ubiquitination of Cytokine Signaling Components
E3s are a subset of the CRL (Cullin-Ring-Ligase) family of ubiquitin ligases with the SOCS protein acting as the substrate recruitment module and interacting specifically with Cullin5, the E3 scaffold. Included here are protocols for the expression
技术资料暂无技术资料 索取技术资料
