- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-42543h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Nucleoside diphosphate kinase B,NME2
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Nucleoside diphosphate kinase B, NME2 ELISA KIT
Product Name:Human Nucleoside diphosphate kinase B, NME2 ELISA KIT
Packing:96T
Catalog No.:ELI-42543h
Gene Name:NME2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Human Nucleoside diphosphate kinase B
Alternative Name:NME2,Human Nucleoside diphosphate kinase B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Nucleoside diphosphate kinase B, NME2 ELISA KIT allows for the in vitro quantitative determination ofHuman Nucleoside diphosphate kinase B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Nucleoside diphosphate kinase B, NME2 ELISA KIT has been pre-coated with an NME2 antibody specific to Human Nucleoside diphosphate kinase B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Nucleoside diphosphate kinase B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Nucleoside diphosphate kinase B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Human Nucleoside diphosphate kinase B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Manning, G., Whyte, D.B., Martinez, R., Hunter, T., and Sudarsanam, S. 2002b. The protein kinase complement of the human genome. Science 298:1912‐1934.
【分享】Human press METHODS IN MOLECULAR BIOLOGY
and Protocols. Edited by Pascol Bailon, 2000 148.DNA-protein interactions, Principle and protocols, 2nd, Edited by Tom Moss, 2000 149.The ELISA guide book, Edited by John Crowther, 2000 150.Complement, Methods and protocols. Edited by B. Paul Morgan. 2000
. shilei5794749 呵呵，这个ELISA检测基因突变比较意思。刚刚看了几篇文章，跟楼主分享一下。 文章：Detection of four b-thalassemia point mutations in Iranians using a PCR-ELISA genotyping system. 检测图中四个位置的基因突变。这个检测方法的四要素：a，DIG labed PCR products b，5’biotinylated
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