- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-35519h
- 2026年01月04日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Group IIE secretory phospholipase A2,PLA2G2E
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human PLA2G2E (Group IIE secretory phospholipase A2) ELISA Kit (CUSTOM)
96 Tests
Operating instructions
FOR RESEARCH USE ONLY!
Alternative name
Human PLA2G2E ELISA KIT ,Human GIIE sPLA2 ELISA KIT ,Human sPLA2-IIE ELISA KIT ,Human phosphatidylcholine 2-acylhydrolase 2E ELISA KIT ,Human phospholipase A2 ELISA KIT ,Human group IIE ELISA KIT ,Human phospholipase A2 group IIE ELISA KIT
Intended use
Human PLA2G2E ELISA kit allows for the in vitro quantitative determination of Human Group IIE secretory phospholipase A2 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
Human PLA2G2E ELISA kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLA2G2E, During the reaction, PLA2G2E in the sample or standard competes with a fixed amount of biotin-labeled PLA2G2E for sites on a pre-coated Monoclonal antibody specific to PLA2G2E. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PLA2G2E in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Customized product Min.Order 5kits
Delivery time 4-6month
Order Human PLA2G2E ELISA kit by email:
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文献和实验Purification and Assay of Mammalian Group I and Group IIa Secretory Phospholipase A2
Phospholipases A2 (PLA2 ) are a family of ubiquitous lipolytic enzymes that are found both as intracellular and secreted proteins. Secretory PLA2 s are small (14 kDa), homologous proteins that require millimolar Ca2+ for catalytic activity
Efficient Immobilization of Phospholipase A2
Immobilized enzymes have been widely used for biomedical and industrial applications. Since phospholipase A2 (PLA2 ; E.C.3.1.1.4) catalyzes the hydrolysis of a wide range of phospholipid aggregates, immobilized PLA2 should also have potential
formation (2 ) and specific eicosanoids seem to be produced by specific cell types in response to various inflammatory stimuli (3 ). The multiple synthetic pathways suggest that multiple sites of inhibition may exist. Phospholipase A2 (PLA2 ) acts on cell
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