- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-42261h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Inter-alpha-trypsin inhibitor heavy chain H3,ITIH3 ELIS
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Inter- alpha- trypsin inhibitor heavy chain H3, ITIH3 ELISA KIT
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
Inter-alpha-trypsin inhibitor heavy chain H3,ITI heavy chain H3,ITI-HC3,Inter-alpha-inhibitor heavy chain 3, ITIH3,H3P; ITI heavy chain H3; ITI-HC3; SHAP; inter-alpha (globulin) inhibitor H3; inter-alpha (globulin) inhibitor, H3 polypeptide; inter-alpha-inhibitor heavy chain 3; inter-alpha-trypsin inhibitor heavy chain H3; pre-alpha (globulin) inhibitor, H3 polypeptide; serum-derived hyaluronan-associated protein; inter-alpha-trypsin inhibitor heavy chain 3
Search name
Human Inter-alpha-trypsin inhibitor heavy chain H3 ELISA KIT ,Human ITI heavy chain H3 ELISA KIT ,Human ITI-HC3 ELISA KIT ,Human Inter-alpha-inhibitor heavy chain 3 ELISA KIT ,Human ITIH3 ELISA KIT ,Human H3P ELISA KIT ,Human ITI heavy chain H3 ELISA KIT ,Human ITI-HC3 ELISA KIT ,Human SHAP ELISA KIT ,Human inter-alpha (globulin) inhibitor H3 ELISA KIT ,Human inter-alpha (globulin) inhibitor ELISA KIT ,Human H3 polypeptide ELISA KIT ,Human inter-alpha-inhibitor heavy chain 3 ELISA KIT ,Human inter-alpha-trypsin inhibitor heavy chain H3 ELISA KIT ,Human pre-alpha (globulin) inhibitor ELISA KIT ,Human H3 polypeptide ELISA KIT ,Human serum-derived hyaluronan-associated protein ELISA KIT ,Human inter-alpha-trypsin inhibitor heavy chain 3 ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of human inter-alpha-trypsin inhibitor heavy chain h3,ITIH3 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ITIH3, During the reaction, ITIH3 in the sample or standard competes with a fixed amount of biotin-labeled ITIH3 for sites on a pre-coated Monoclonal antibody specific to ITIH3. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of ITIH3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PDF Manual download
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大学和汉阳大学的研究人员发现 G 蛋白α- 13（Gα13）的表达在糖尿病小鼠和人类的肝脏中下降了。在小鼠中仅剔除肝脏中的 Gna13 基因可导致全身性葡萄糖耐受不良。本研究表明小鼠中的全身胰岛素抵抗是由 Gα13 下调导致的肝脏中 ITIH1 过度分泌引起的，这可通过 ITIH1 中和加以逆转。这些发现可能有助于开发治疗代谢疾病的新策略。原文检索：Overproduction of inter-α-trypsin inhibitor heavy chain 1 after loss of Gα13
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