- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 进口
- ELI-40105h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Activating signal cointegrator 1,TRIP4
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Activating signal cointegrator 1, TRIP4 ELISA KIT
Product Name:Human Activating signal cointegrator 1, TRIP4 ELISA KIT
Packing:96T
Catalog No.:ELI-40105h
Gene Name:TRIP4
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Activating signal cointegrator 1(TRIP4
Alternative Name:TRIP4,Activating signal cointegrator 1(TRIP4
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Activating signal cointegrator 1, TRIP4 ELISA KIT allows for the in vitro quantitative determination of Activating signal cointegrator 1(TRIP4 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Activating signal cointegrator 1, TRIP4 ELISA KIT has been pre-coated with an Activating signal cointegrator 1(TRIP4 antibody specific to Activating signal cointegrator 1(TRIP4 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Activating signal cointegrator 1(TRIP4 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Activating signal cointegrator 1(TRIP4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Activating signal cointegrator 1(TRIP4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验should be analyzed. Protein levels can be monitored by Western blot, immunofluorescence, ELISA or other means. (Protein can be isolated using Ambion's PARIS™ Kit.) For quantitating mRNA levels, however, qRT-PCR is again the preferred choice.An example
Microarray Analysis of DNA Replication Timing
here for generation of high-resolution temporal maps of DNA replication in synchronized human cells. This method does not require amplification of DNA before microarray hybridization and so avoids errors introduced during PCR. A major advantage of using this procedure
). 3ZF proteins may be capable of binding multiple sites in the human genome and activating, to varying degrees, more than one gene. Depending on the application, this could be a limitation or an advantage. Table 1: 6ZF (top) and 3ZF
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