- ￥2890
- LSM Bio
- 0.312-20ng/ml
- 0.1ng/ml
- 进口
- ELI-39607h
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.312-20ng/ml
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Leucyl-tRNA synthetase,cytoplasmic,LARS
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/ml
- 规格：
96T
Human Leucyl- tRNA synthetase, cytoplasmic, LARS ELISA KIT
Product Name:Human Leucyl- tRNA synthetase, cytoplasmic, LARS ELISA KIT
Packing:96T
Catalog No.:ELI-39607h
Gene Name:LARS
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Leucyl-tRNA synthetase(cytoplasmic(LARS
Alternative Name:LARS,Leucyl-tRNA synthetase(cytoplasmic(LARS
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Leucyl- tRNA synthetase, cytoplasmic, LARS ELISA KIT allows for the in vitro quantitative determination of Leucyl-tRNA synthetase(cytoplasmic(LARS concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Leucyl- tRNA synthetase, cytoplasmic, LARS ELISA KIT has been pre-coated with an Leucyl-tRNA synthetase(cytoplasmic(LARS antibody specific to Leucyl-tRNA synthetase(cytoplasmic(LARS .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Leucyl-tRNA synthetase(cytoplasmic(LARS and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Leucyl-tRNA synthetase(cytoplasmic(LARS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Leucyl-tRNA synthetase(cytoplasmic(LARS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验有替代前者的趋势。由于ABS-ELISA较普通ELISA多用了两种试剂，增加了操作步骤，在临床检验中ABS-ELISA应用不多。科研项目中检测微量的成分如细胞因子常采用本法。晶美分装ELISA KIT采用的方法：1， TORCH及传染病试剂盒（间接法），见2.2.32， TORCH-IgM捕获法特色：包被抗体，标记抗原原理：3， 细胞因子试剂盒采用的方法路线（ABC-ELISA）原理产品特色：采用ABC法，灵敏度更高，特异性更强。生物素抗体和酶联物是浓缩的，使用前需用相应的缓冲液稀释。酶联物可以通用
的原理用于分子诊断方面：TrimGen公司的KRAS Mutation Detection Kit在96孔板里一次能实现7/8个KRAS突变位点的检测。 另外基于相同原理的技术还有PPT-ELISA。这是用来筛截短突变的，相关文章见：1，Rapid screen for truncating ATM mutations by PTT-ELISA . 2，An ELISA-based high throughput protein truncation test
时对一个样本中的75个目的蛋白进行精确定量。2、样本需要量小。仅需要50μL溶液就可进行一次检测。3、更高的灵敏度。分析灵敏度高达2.8pg/mL。4、更高的灵敏度、更宽的检测范围和更好的重复性。CBA技术使蛋白定量的分析灵敏度高达pg/ml级别,检测范围达0-5000pg/ml，所有分析只需一组标准曲线。根据抗体的荧光强度对目的蛋白定量，排除了ELISA反应中酶联放大产生的假阳性。5、检测对象的灵活组合—BD CBA Flex Set。CBA kit是同时检测试剂盒配好的6个指标，形式比较固定
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