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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10NG/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human CD100
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078NG/ML
- 规格:
96T
CD100 ELISA KIT|Human
Catalog EF008487
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
CD100 ELISA KIT Sensitivity< 0.188ng/ml
Application CD100 ELISA KIT|Human is for detecting the concentration of Human CD100 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of CD100 ELISA KIT|Human
The microtiter plate provided in CD100 ELISA KIT|Human has been pre-coated with an Human CD100 antibody specific to Human CD100. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human CD100antibody preparation specific for Human CD100 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human CD100, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human CD100in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that CD100 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验This protocol is based on enrichment from 1 x 107 leucocytes (MNC). It is scalable from 1 x 107 –2 x 109 cells. Keep the cells and buffers cold (2–8°C) during the whole process. 1) Transfer 100 μl (1 x 107 ) leucocytes in Buffer
大鼠CD4分子(CD4) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中 CD4分子(CD4) 含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 大鼠 CD4分子( CD4 ) 水平。用纯化的 大鼠 CD4分子( CD4 ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 CD4分子( CD4 ) ,再与HRP
人 CD147 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中 CD147 的 含量。 实验原理 : 本试剂盒应用 双抗体 夹心法测定 标本 中 人 CD147 水平。用纯化的 人 CD147 抗体 包被微孔板,制成固相 抗 体,往包被 单抗 的微孔中依次加入 CD147 ,再与HRP 标记的 CD147 抗体结合,形成抗体- 抗原 - 酶标
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