- ￥2890
- LSM Bio
- 0.31-20ng/Ml
- 0.1ng/Ml
- 进口
- ELI-33983h
- 2026年03月16日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.31-20ng/Ml
- 检测方法：
夹心法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human T-cell surface glycoprotein CD3 zeta,CD247
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.1ng/Ml
- 规格：
96T
Human T- cell surface glycoprotein CD3 zeta, CD247 ELISA Kit
96 Tests
Operating instructions
FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!
Synonyms
CD247,CD247 molecule; CD247 antigen, zeta subunit; CD3-ZETA; CD3H; CD3Q; CD3Z; T3Z; TCRZ; CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 zeta chain; T-cell surface glycoprotein CD3 zeta chain; TCR zeta chain
Search name
Human CD247 ELISA KIT ,Human CD247 molecule ELISA KIT ,Human CD247 antigen zeta subunit ELISA KIT ,Human CD247 antigen ELISA KIT ,Human CD3-ZETA ELISA KIT ,Human CD3H ELISA KIT ,Human CD3Q ELISA KIT ,Human CD3Z ELISA KIT ,Human T3Z ELISA KIT ,Human TCRZ ELISA KIT ,Human CD3Z antigen ELISA KIT ,Human zeta polypeptide (TiT3 complex) ELISA KIT ,Human CD3zeta chain ELISA KIT ,Human T-cell antigen receptor complex ELISA KIT ,Human zeta subunit of CD3 ELISA KIT ,Human T-cell receptor T3 zeta chain ELISA KIT ,Human T-cell surface glycoprotein CD3 zeta chain ELISA KIT ,Human TCR zeta chain ELISA KIT ,Human T-cell surface glycoprotein CD3 zeta ELISA KIT
Intended use
This immunoassay kit allows for the in vitro quantitative determination of Human T- cell surface glycoprotein CD3 zeta, CD247 concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Test principle
The ELISA is based on the competitive binding enzyme immunoassay technique.The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human T- cell surface glycoprotein CD3 zeta, CD247, During the reaction, Human T- cell surface glycoprotein CD3 zeta, CD247 in the sample or standard competes with a fixed amount of biotin-labeled Human T- cell surface glycoprotein CD3 zeta, CD247 for sites on a pre-coated Monoclonal antibody specific to Human T- cell surface glycoprotein CD3 zeta, CD247. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human T- cell surface glycoprotein CD3 zeta, CD247 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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文献和实验上 Strep-tag 的 Fab 片段（Fab-Streps）特异性的结合到琼脂糖基质上。随后，加入全血或其他血液制剂流过柱子。目的细胞通过特异性的 Fab-Strep 作用从而结合到琼脂糖基质上，其他非目的细胞则有效的被洗去。最后，加入生物素使目的细胞从 Fab-Streps 上解离。至此，就已分离得到不带任何标记的、真实的目的细胞可用于下游的实验研究。 1. 实验流程 所需产品： CD3 Fab-TACS® Gravity Kit, human（IBA Lifesciences, Cat
及释放目标细胞。以不具磁性且可逆的试剂，取代一般具高亲和力的完整抗体，从而达到直接由全血或其他样本，进行温和标准化正选的目的。Fab-TACS 正选，化解了传统使用完整抗体进行正选的困境，其对目标细胞无不必要的刺激，避免了分选流程的细胞活化，也避免完整抗体与特定受体的结合而影响的分析准确度。因此，使用 Fab-TACS 无痕正选技术可富集不含标记、未激活的目标细胞，次次精准。人全血 CD3+ T 细胞分选实例1. 所需产品：(1) CD3 Fab-TACS® Gravity Kit, human
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
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