- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078ng/ml
- 国产
- ELA-E4373h
- 2025年07月07日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Rho GTPase Activating Protein 21
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Rho GTPase Activating Protein 21 ELISA Kit
Product Name:Human Rho GTPase Activating Protein 21 ELISA Kit
Packing:96T
Catalog No.:ELA-E4373h
Gene Name:ARHGAP21
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Rho GTPase Activating Protein 21
Alternative Name:ARHGAP21,Rho GTPase Activating Protein 21
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Rho GTPase Activating Protein 21 ELISA Kit allows for the in vitro quantitative determination of Rho GTPase Activating Protein 21 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Rho GTPase Activating Protein 21 ELISA Kit has been pre-coated with an Rho GTPase Activating Protein 21 antibody specific to Rho GTPase Activating Protein 21 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Rho GTPase Activating Protein 21 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Rho GTPase Activating Protein 21, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Rho GTPase Activating Protein 21 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Identification and Characterization of Small GTPase-Associated Kinases
. Here we describe a method that was developed to analyze p21-interacting proteins in total protein extracts after their separation on sodium dodecyl sulfate (SDS)-polyacrylamide gels. In fact, both guanosine 5′ triphosphatase (GTPase)-activating proteins and GTPase
Biochemical Assays to Characterize Rho GTPases
. These very slow reactions require catalysis in cells by two kinds of regulatory proteins. While the guanine nucleotide exchange factors (GEFs) activate small GTPases by stimulating the exchange of bound GDP for the cellular abundant GTP, GTPase-activating proteins
小GTP结合蛋白(small GTP binding protein)
), 它催化GDT同GTP的交换。另一种是GTP 酶激活蛋白(GTPase-activating protein, GAP), 它触发结合的GTP水解。 许多小GTP结合蛋白都有一个共价结合的脂基团, 帮助它们同膜结合。小GTP蛋白包括ras、rab和rho蛋白, 它们在一些重要的的活动中起调节作用, 包括分泌活动和细胞骨架的装配等。
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