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- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.31-20ng/Ml
- 检测方法:
夹心法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Cyclin-dependent kinase 12,CDK12
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.1ng/Ml
- 规格:
96T
Human Cyclin- dependent kinase 12, CDK12 ELISA KIT
Product Name:Human Cyclin- dependent kinase 12, CDK12 ELISA KIT
Packing:96T
Catalog No.:ELI-33852h
Gene Name:CDK12
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cyclin-dependent kinase 12(CDK12
Alternative Name:CDK12,Cyclin-dependent kinase 12(CDK12
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cyclin- dependent kinase 12, CDK12 ELISA KIT allows for the in vitro quantitative determination of Cyclin-dependent kinase 12(CDK12 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cyclin- dependent kinase 12, CDK12 ELISA KIT has been pre-coated with an Cyclin-dependent kinase 12(CDK12 antibody specific to Cyclin-dependent kinase 12(CDK12 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cyclin-dependent kinase 12(CDK12 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-dependent kinase 12(CDK12, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cyclin-dependent kinase 12(CDK12 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand break repair and V(D)J recombination (1 –3 ). DNA-PK is composed of a large catalytic subunit of approx 460 kDa (DNA-PKcs) and a heterodimeric DNA targeting subunit
RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
低至0.12 ng/mL灵敏度,Elabscience未包被人内皮抑素(ES) elisa试剂盒精准捕捉低丰度样本变化
试剂盒 货号:E-UNEL-H0382 规格:96 T × 5 / 96 T × 15 反应性:人(Human) 检测范围:0.16 - 10 ng/mL 灵敏度:0.12 ng/mL 样本类型:血清、血浆或其他生物体液 检测方法:双抗体夹心法(Sandwich-ELISA),比色法检测 反应时间:24小时 储存与有效期:-20℃保存,有效期12个月 检测原理:双抗体夹心法定量分析 未包被人内皮抑素(ES) elisa试剂盒采用经典的双抗体夹心ELISA原理: 包被
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