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- 详细信息
- 文献和实验
- 技术资料
- 库存:
20
- 供应商:
LSMBIO
- 检测范围:
0.156-10ng/ML
- 检测方法:
夹心法
- 应用:
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Human Cadherin-like protein 26,CDH26
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078 ng/Ml
- 规格:
96T
Human Cadherin- like protein 26, CDH26 ELISA KIT
Product Name:Human Cadherin- like protein 26, CDH26 ELISA KIT
Packing:96T
Catalog No.:ELI-49270h
Gene Name:CDH26
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cadherin-like protein 26(CDH26
Alternative Name:CDH26,Cadherin-like protein 26(CDH26
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cadherin- like protein 26, CDH26 ELISA KIT allows for the in vitro quantitative determination of Cadherin-like protein 26(CDH26 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cadherin- like protein 26, CDH26 ELISA KIT has been pre-coated with an Cadherin-like protein 26(CDH26 antibody specific to Cadherin-like protein 26(CDH26 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cadherin-like protein 26(CDH26 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cadherin-like protein 26(CDH26, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cadherin-like protein 26(CDH26 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
μL) into new 1.5 ml centrifuge tube for RNA/DNA isolation. Remove any liquid from the original tube contains protein precipitate by invert the plate on a absorbent paper. Keep the tube contains protein pellet for protein isolation start from step 26
Using Cadherin Expression to Assess Spontaneous Differentiation of Embryonic Stem Cells
- to N-cadherin switch occurs during spontaneous differentiation of both murine and human ESCs. Here we describe the use of E-cadherin and N-cadherin proteins and transcript expression for assessing the proportion of undifferentiated and spontaneously
human E-cadherin-IgG Fc domain fusion protein (E-cad-Fc) has recently been shown to be extremely efficient in facilitating the culture of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells under completely defined conditions
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