- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产(香港)
- ELI-27649h
- 2026年05月21日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Eukaryotic translation initiation factor 1,EIF1
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Eukaryotic translation initiation factor 1, EIF1 ELISA KIT
Product Name:Human Eukaryotic translation initiation factor 1, EIF1 ELISA KIT
Packing:96T
Catalog No.:ELI-27649h
Gene Name:EIF1
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Eukaryotic translation initiation factor 1(EIF1
Alternative Name:EIF1,Eukaryotic translation initiation factor 1(EIF1
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Eukaryotic translation initiation factor 1, EIF1 ELISA KIT allows for the in vitro quantitative determination of Eukaryotic translation initiation factor 1(EIF1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Eukaryotic translation initiation factor 1, EIF1 ELISA KIT has been pre-coated with an Eukaryotic translation initiation factor 1(EIF1 antibody specific to Eukaryotic translation initiation factor 1(EIF1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Eukaryotic translation initiation factor 1(EIF1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Eukaryotic translation initiation factor 1(EIF1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Eukaryotic translation initiation factor 1(EIF1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Genome‐Wide Annotation and Quantitation of Translation by Ribosome Profiling
reading frames, an adaptation that reveals the sites of translation initiation by pre?treating cells with harringtonine to immobilize initiating ribosomes is described. The protocol described requires 5 to 7 days to generate a completed ribosome profiling
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
translation initiation factor (eIF) 2 (a G protein) functions in its GTP-bound state to deliver initiator methionyl-tRNA (tRNAiMet) to the small ribosomal subunit and is necessary for protein synthesis in all cells1, 2. Phosphorylation of eIF2 [eIF2(αP
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