Target Protein Name:Carbonyl reductase [NADPH] 1(CBR1
Alternative Name:CBR1,Carbonyl reductase [NADPH] 1(CBR1 Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit Product Description:Human Carbonyl reductase [NADPH] 1, CBR1 ELISA KIT allows for the in vitro quantitative determination of Carbonyl reductase [NADPH] 1(CBR1 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle: The microtiter plate provided in Human Carbonyl reductase [NADPH] 1, CBR1 ELISA KIT has been pre-coated with an Carbonyl reductase [NADPH] 1(CBR1 antibody specific to Carbonyl reductase [NADPH] 1(CBR1 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Carbonyl reductase [NADPH] 1(CBR1 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Carbonyl reductase [NADPH] 1(CBR1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Carbonyl reductase [NADPH] 1(CBR1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!