- ￥2890
- LSM Bio
- 0.156-10ng/ml
- 0.078ng/ml
- 国产
- ELI-21580h
- 2025年06月26日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.156-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Ribosomal RNA-processing protein 8,RRP8
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078ng/ml
- 规格：
96T
Human Ribosomal RNA- processing protein 8, RRP8 ELISA KIT
Product Name:Human Ribosomal RNA- processing protein 8, RRP8 ELISA KIT
Packing:96T
Catalog No.:ELI-21580h
Gene Name:RRP8
Detect Range:78-5000 pg/mL
Sensitivity:32pg/mL
Target Protein Name:Ribosomal RNA-processing protein 8(RRP8
Alternative Name:RRP8,Ribosomal RNA-processing protein 8(RRP8
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Ribosomal RNA- processing protein 8, RRP8 ELISA KIT allows for the in vitro quantitative determination of Ribosomal RNA-processing protein 8(RRP8 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Ribosomal RNA- processing protein 8, RRP8 ELISA KIT has been pre-coated with an Ribosomal RNA-processing protein 8(RRP8 antibody specific to Ribosomal RNA-processing protein 8(RRP8 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Ribosomal RNA-processing protein 8(RRP8 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Ribosomal RNA-processing protein 8(RRP8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Ribosomal RNA-processing protein 8(RRP8 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Combine the RNA and gene-specific primers with the kit components, and the entire reaction is ready. The Access System allows the most control over the reaction conditions, and the AccessQuick™ System offers the most convenience. Both use the robust AMV
Isolate It All：siRNA • miRNA • Total RNA • Native Protein
• Isolate total RNA and native protein from the same sample • Enrich for small RNAs ( • Ideal for assessing siRNA or miRNA expression and processing Small interfering RNAs (siRNAs) and microRNAs (miRNAs
Methods to Remove DNA Contamination from RNA Samples
, for the presence of ribosomal protein S15 message. Regardless of whether contaminating DNA was visible by gel assessment, both RNA samples showed amplifiable DNA in the no-RT control (Figure 1b). Once DNA contamination was verified, both RNA samples were subjected
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