- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 国产(香港)
- EF006917
- 2026年05月21日
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- 文献和实验
- 技术资料
- 库存：
20
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human HSP gp96
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
HSP gp96 ELISA KIT|Human
Catalog EF006917
Packing 48T/96T
Reactivity Human
Detect Range 0.313-20ng/ml
HSP gp96 ELISA KIT Sensitivity< 0.188ng/ml
Application HSP gp96 ELISA KIT|Human is for detecting the concentration of Human HSP gp96 in serum, plasma, tissue homogenates and other biological fluids.
Storage condition 6 month at 4 Centigrade
Principle of HSP gp96 ELISA KIT|Human
The microtiter plate provided in HSP gp96 ELISA KIT|Human has been pre-coated with an Human HSP gp96 antibody specific to Human HSP gp96 . Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human HSP gp96 antibody preparation specific for Human HSP gp96 then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human HSP gp96 , biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human HSP gp96 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
*Please noted that HSP gp96 ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!
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文献和实验Cellular stress proteins like the classical heat-shock proteins (HSPs) hsp70 (1 –3 ), hsp90 (3 ) and hsp110 (4 ); the glucose-regulated proteins gp96/GRP94 (3 ,5 ) and grp170 (4 ); as well as the endoplasmic chaperone calreticulin
调至 1μg/μl。 4. ELISA 检测：按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数，结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理，全程低温操作，裂解液现配现用； 2. 蛋白定量时标准品与样本同步孵育，稀释采用倍比稀释减少误差； 3. ELISA 实验需每次重绘标准曲线，洗板充分、严格把控显色与终止时间。 免疫检测试剂盒： 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
with different molecular weights and different intracellular localizations. These proteins undertake crucial functions in maintaining cell homeostasis, and therefore they have been conserved during evolution. Hsp70 and Grp94/gp96, due to their peptide chaperone
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