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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
参照说明书
- 检测方法:
夹心法/竞争法
- 应用:
0.1ng/ml
- 适应物种:
人
- 标记物:
Cyclin- dependent kinase- like 2, CDKL2
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.31-20ng/ml
- 规格:
96T
Human Cyclin- dependent kinase- like 2, CDKL2 ELISA KIT
Product Name:Human Cyclin- dependent kinase- like 2, CDKL2 ELISA KIT
Packing:96T
Catalog No.:ELI-11038h
Gene Name:CDKL2
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:Cyclin-dependent kinase-like 2(CDKL2
Alternative Name:CDKL2,Cyclin-dependent kinase-like 2(CDKL2
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Cyclin- dependent kinase- like 2, CDKL2 ELISA KIT allows for the in vitro quantitative determination of Cyclin-dependent kinase-like 2(CDKL2 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Cyclin- dependent kinase- like 2, CDKL2 ELISA KIT has been pre-coated with an Cyclin-dependent kinase-like 2(CDKL2 antibody specific to Cyclin-dependent kinase-like 2(CDKL2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Cyclin-dependent kinase-like 2(CDKL2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Cyclin-dependent kinase-like 2(CDKL2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Cyclin-dependent kinase-like 2(CDKL2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验【分享】Human press METHODS IN MOLECULAR BIOLOGY
Cyclins, Cyclin-Dependent Kinases, and Other Factors Edited by b Giordano, Antonio 2004 286.Transgenic Plants Methods and Protocols. Edited by Peña, Leandro 2004 287.Epigenetics Protocols. Edited by Tollefsbol, Trygve O. 2004 288.Oligonucleotide
and CDK5/p25, two protein kinases involved in abnormal tau phosphorylationin Alzheimer’s disease. A propertycommon to most cyclin-dependent kinase inhibitors? J Biol Chem. 276 , 251–260. 20. Leost M, Schultz C, Link A, et al. (2000)Paullones
, and Elledge SJ. (2002). Regulation of ATR substrate selection by Rad17-dependent loading of Rad9 complexes onto chromatin. Genes & Devel. 16:198-208. 8. Stucke VM, Sillje HHW, Arnaud L, and Nigg EA. (2002). Human Mps1 kinase is required for the spindle
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