Human Glucagon- like peptide 1

Human Glucagon- like peptide 1 receptor, GLP1R ELISA KIT

96 Tests

Operating instruction

FOR RESEARCH USE ONLY; 

Alternative name

Human GLP1R ELISA KIT ,Human glucagon-like peptide 1 receptor ELISA KIT ,Human GLP-1 receptor ELISA KIT ,Human GLP-1-R ELISA KIT ,Human GLP-1R ELISA KIT,Human exendin 4 ELISA KIT,Human GLP-1 receptor agonist ELISA KIT

Intended use

Human GLP1R ELISA KIT allows for the in vitro quantitative determination of human Glucagon-like peptide 1 receptor,GLP-1 receptor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in this kit has been pre-coated with an antibody specific to GLP-1 receptor. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for GLP-1 receptor and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain GLP-1 receptor, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GLP-1 receptor in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range:0.156-10ng/ml

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