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- 详细信息
- 文献和实验
- 技术资料
- 库存:
50
- 供应商:
LSMBIO
- 检测范围:
0.15-10ng/ml
- 检测方法:
夹心法/竞争法
- 应用:
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种:
人
- 标记物:
Phospholipase A2, PLA2G1B
- 样本:
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度:
0.078ng/ml
- 规格:
96T
Human Phospholipase A2, PLA2G1B ELISA KIT
Product Name:Human Phospholipase A2, PLA2G1B ELISA KIT
Packing:96T
Catalog No.:ELI-02056h
Gene Name:PLA2G1B
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Phospholipase A2(PLA2G1B
Alternative Name:PLA2G1B,Phospholipase A2(PLA2G1B
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Phospholipase A2, PLA2G1B ELISA KIT allows for the in vitro quantitative determination of Phospholipase A2(PLA2G1B concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Phospholipase A2, PLA2G1B ELISA KIT has been pre-coated with an Phospholipase A2(PLA2G1B antibody specific to Phospholipase A2(PLA2G1B .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Phospholipase A2(PLA2G1B and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Phospholipase A2(PLA2G1B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Phospholipase A2(PLA2G1B in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验Efficient Immobilization of Phospholipase A2
Immobilized enzymes have been widely used for biomedical and industrial applications. Since phospholipase A2 (PLA2 ; E.C.3.1.1.4) catalyzes the hydrolysis of a wide range of phospholipid aggregates, immobilized PLA2 should also have potential
Introduction of human 14-3-3 proteins
whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
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