- ￥2890
- LSM Bio
- 0.15-10NG/ML
- 0.078NG/ML
- 进口
- ELI-50307h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10NG/ML
- 检测方法：
夹心法
- 应用：
夹心法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
Human Histone H3-like centromeric protein A,CENPA
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078NG/ML
- 规格：
96T
Human Histone H3- like centromeric protein A, CENPA ELISA KIT
Product Name:Human Histone H3- like centromeric protein A, CENPA ELISA KIT
Packing:96T
Catalog No.:ELI-50307h
Gene Name:CENPA
Detect Range:0.312-20 ng/mL
Sensitivity:0.039ng/mL
Target Protein Name:Histone H3-like centromeric protein A(CENPA
Alternative Name:CENPA,Histone H3-like centromeric protein A(CENPA
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human Histone H3- like centromeric protein A, CENPA ELISA KIT allows for the in vitro quantitative determination of Histone H3-like centromeric protein A(CENPA concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human Histone H3- like centromeric protein A, CENPA ELISA KIT has been pre-coated with an Histone H3-like centromeric protein A(CENPA antibody specific to Histone H3-like centromeric protein A(CENPA .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Histone H3-like centromeric protein A(CENPA and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Histone H3-like centromeric protein A(CENPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of Histone H3-like centromeric protein A(CENPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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文献和实验1a).Part II Triple immunoprecipitation: H3K27 ChIP/-flag-DNMT3A/biotin-RNA A total of 4.0×106 293T cells are plated out and 24 hours later transfected plated (~70% confluent at the time of transfection) with the flag-tagged protein expressing plasmid
Identification of Protein Interactions by Far Western Analysis
protein of interest cloned into an in vitro expression vector In vitro transcription/translation kit (Promega) 10 mCi/ml 35
(Figure 1a). Part II Triple immunoprecipitation: H3K27 ChIP/-flag-DNMT3A/biotin-RNA A total of 4.0×106 293T cells are plated out and 24 hours later transfected plated (~70% confluent at the time of transfection) with the flag-tagged protein
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