- ￥2890
- LSM Bio
- 0.15-10ng/ml
- 0.078mg/ml
- 国产
- ELA-E0616h
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 供应商：
LSMBIO
- 检测范围：
0.15-10ng/ml
- 检测方法：
夹心法/竞争法
- 应用：
夹心法/竞争法ELISA体外定量检测人血清、血浆或其它相关生物液体中蛋白浓度
- 适应物种：
人
- 标记物：
nuclear factor- kappaB p65
- 样本：
人血清、血浆或其它相关生物液体中天然和重组蛋白
- 灵敏度：
0.078mg/ml
- 规格：
96T
Human nuclear factor- kappaB p65 ELISA Kit
Product Name:Human nuclear factor- kappaB p65 ELISA Kit
Packing:96T
Catalog No.:ELA-E0616h
Gene Name:NF-kappaB p65
Detect Range:0.156-10 ng/mL
Sensitivity:0.078ng/mL
Target Protein Name:nuclear factor-kappaB p65
Alternative Name:NF-kappaB p65,nuclear factor-kappaB p65
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA type:Sandwich ELISA Kit
Product Description:Human nuclear factor- kappaB p65 ELISA Kit allows for the in vitro quantitative determination of nuclear factor-kappaB p65 concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.
ELISA Test Principle:
The microtiter plate provided in Human nuclear factor- kappaB p65 ELISA Kit has been pre-coated with an nuclear factor-kappaB p65 antibody specific to nuclear factor-kappaB p65 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for nuclear factor-kappaB p65 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain nuclear factor-kappaB p65, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.The concentration of nuclear factor-kappaB p65 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!
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Nuclear factor-κB (NF-κB) is a key transcription factor that regulates the expression of genes involved in immune and inflammatory responses and in cell death and survival. This chapter describes in detail the method for measuring the NF-κB
immunoprecipitation (ChIP) to analyze the regulation of NFκB recruitment to NFκB-dependent promoters by nuclear IκBα induced by LMB. We show that in lipopolysaccharide (LPS)-stimulated human U-937 macrophages, recruitment of NFκB p65 and p50 proteins to NFκB-dependent
【求助】药物处理后细胞凋亡，但RT-PCR结果显示c-myc上调，结果奇怪？
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