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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
thioredoxin
- 亚型:
IgG
- 形态:
liquid
- 保存条件:
负20摄氏度
- 克隆性:
Polyclonal antibody
- 标记物:
Non-conjugated
- 适应物种:
Human,Mouse ,Rat
- 保质期:
6个月
- 抗原来源:
Rabbit
- 目录编号:
Q9BZJ3
- 级别:
纯化级别
- 库存:
50
- 供应商:
LSM bio
- 宿主:
E. coli - derived recombinant protein
- 应用范围:
ELISA,WB,IHC,IF,FC
- 浓度:
≥95% as determined by SDS-PAGE
- 靶点:
thioredoxin
- 抗体英文名:
anti-TRX antibody,TRX antibody
- 抗体名:
anti-TRX 抗体,TRX 抗体
- 规格:
100μl
TRX抗体| TRX antibody
货号 PAab09033
蛋白别名 ADF antibody, ATL derived factor antibody, DKFZp686B1993 antibody, SASP antibody, Thioredoxin antibody, TRDX antibody, TRX antibody, TRX1 antibody, TXN
蛋白介绍
TXN, TRDX, TRX, TRX1, ADF and SASP, belongs to the thioredoxin family. It participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. TXN plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity. TXN induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity.
产品描述
anti-TRX antibody is a Rabbit Polyclonal antibody againstTRX..
建议稀释比例
WB:1:1000-1:10000;IP:1:200-1:1000;IHC:1:100-1:400;IF:1:20-1:200
IHC
Immunohistochemistry of paraffin-embedded human liver tissue slide using PAab09033( TXN Antibody) at dilution of 1:200
Western blot
Jurkat cells were subjected to SDS PAGE followed by western blot with PAab09033( TXN Antibody) at dilution of 1:2000(本抗体仅供体外科研用途,不可用于临床诊断!) "">
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文献和实验人硫氧化还原蛋白(Trx) 酶联免疫分析( ELISA ) 试剂盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定人血清,血浆及相关液体样本中硫氧化还原蛋白(Trx) 的 含量。 实验原理: 本试剂盒应用双抗体夹心法测定标本中人硫氧化还原蛋白 (Trx) 水平。用纯化的人硫氧化还原蛋白 (Trx) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入硫氧化还原蛋白 (Trx) , 再与 HRP 标记
Thioredoxins (Trx) are a ubiquitous family of proteins that modulate the enzymatic activity of their substrate proteins by redox regulation. This is achieved by reduction of a disulfide bond within their target proteins. A conserved pair
. 鉴于以上原因,本实验将融合蛋白Trx-PrPC 27-30表达分成两个阶段,前一个阶段为质粒生长阶段,主要保证质粒的稳定性和提高质粒的拷贝数;后一个阶段为融合蛋白表达 阶段,待细菌生长达饱和以后,诱导融合蛋白Trx-PrPC 27-30的表达. 在温度、AMP浓度、培养基等诸多影响质粒PrP-pET-32a(+)稳定性的因素中,以温度影响最为明显.实验结果显示,37℃条件下约60% 以上的细菌无质粒PrP-pET-32a(+),25℃条件下失去质粒PrP-pET-32
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