- ￥2262
- LSM Bio
- 进口
- PAab08638
- 2025年07月17日
- ELISA,IHC
- E. coli - derived recombinant protein
- Human,Mouse ,Rat
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
transforming growth factor, beta 1
- 亚型：
IgG
- 形态：
liquid
- 保存条件：
负20摄氏度
- 克隆性：
Polyclonal antibody
- 标记物：
Non-conjugated
- 适应物种：
Human,Mouse ,Rat
- 保质期：
6个月
- 抗原来源：
Rabbit
- 目录编号：
P67936
- 级别：
纯化级别
- 库存：
50
- 供应商：
LSM bio
- 宿主：
E. coli - derived recombinant protein
- 应用范围：
ELISA,IHC
- 浓度：
≥95% as determined by SDS-PAGE
- 靶点：
transforming growth factor, beta 1
- 抗体英文名：
anti-TGF-beta 1 antibody,TGF-beta 1 antibody
- 抗体名：
anti-TGF-beta 1 抗体,TGF-beta 1 抗体
- 规格：
100μg
TGF-beta 1抗体| TGF-beta 1 antibody
货号 PAab08638
蛋白别名 CED antibody, DPD1 antibody, LAP antibody, TGF beta 1 antibody, TGFB antibody, TGFB1 antibody, TGFbeta antibody, TGF-beta1 antibody, TGF-β1
蛋白介绍
TGFB, also named as LAP and TGFB1, is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for it. TGFB positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. It is highly expressed in bone. Mutation of TGFB are the cause of Camurati-Engelmann disease (CED) which known as progressive diaphyseal dysplasia 1 (DPD1).
产品描述
anti-TGF-beta 1 antibody is a Rabbit Polyclonal antibody againstTGF-beta 1..
建议稀释比例
WB:1:200-1:2000;IHC:1:50-1:500
IHC
Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using PAab08638( TGFB1 Antibody) at dilution of 1:200
Western blot
MCF-7 cells were subjected to SDS PAGE followed by western blot with PAab08638( TGFB1 Antibody) at dilution of 1:600(本抗体仅供体外科研用途，不可用于临床诊断!) "">
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文献和实验Protocol for intracytoplasmic staining of cytokines for FACS analysis
at 37 deg C. Harvest cells and wash into PBS + 0.1% BSA + 0.1% NaN3. 4) Perform any antibody staining of the cell surface that is sensitive to fixation at this stage. If you are using cells with high levels of Fc receptors, and entirely non
【转帖】Top 100 proteomics publications
/ionization mass spectrometry. Expert Rev Proteomics 49. Ji et al. (2006) A sample preparation process for LC-MS/MS analysis of total protein drug concentrations in monkey plasma samples with antibody. J Chromatogr B Analyt Technol Biomed Life Sci 50
/ml，不可振荡。 这个步骤即为溶解步骤，非常重要。 1) 一定要用推荐的溶液重悬(或溶解)冻干粉 用于溶解细胞因子或蛋白的溶液千差万别。此例中的重组人IL-4需用水溶解，而重组人IL-2 (产品编号：200-02)则需用100mM Acetic Acid (醋酸)溶解，重组人TGF-beta1 (产品编号：100-21)需用10mMCitric Acid (柠檬酸)，pH3.0溶解，重组人FGF-basic(产品编号：100-18B)需用5mMTris，pH7.6溶解
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