产品名称:TUNEL原位细胞凋亡检测试剂盒(POD)
英文名称:In Situ Cell Apoptosis Detection Kit, POD
产品货号:GS0247
产品规格:20次|100次
储存条件:冷冻(-20℃)
概述
Apoptosis is essential in many physiological processes.Several methods have been described to identify apoptotic cells.Endonucleolysis is considered as the key biochemical event of apoptosis,resulting in double-stranded,low molecular weight DNA fragments as well as single strand breaks in high molecular weight DNA.Those DNA strand breaks can be identified by labeling free 3’-OH termini with modified nucleotides in an enzymatic reaction.Terminal deoxynucleotidyl transferase(TdT)can catalyze DIG-dUTP to free 3’-OH DNA ends.The DIG-dUTP is first react with biotin-conjugated anti-digoxin antibody,which subsequently associate with streptavidin(SABC).Combined with DAB,apoptotic cells stain yellow analyzed under microscopy.