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文献和实验Genotyping using Affymetrix arrays
) in 100 µL volumes containing 1.0µM of primer (5’-ATT ATG AGC ACG ACA GAC GCC TGA TCT-3’), 0.30 mM dNTPs, 1.0 mM MgSO4, 5 U Platinum® Pfx Polymerase (Invitrogen), PCR Enhancer (Invitrogen) and Pfx Amplification Buffer (Invitrogen). 30 cycles of PCRs
【精华】vol 687 Chapter 4 巢式PCR侧翼序列测定实验
,in which case, proceed to the next step. 5. Use 0.5 ml of product from the preceding step to template anested 50 ml PfuTurbo?hot start PCR in (see Note 13) 1×reaction buffer including 300 mM dNTPs, 3.75 U PfuTurbo® DNA Polymerase, and 250 nM GSP2 and GSP
Geno typing using Affymetrix arrays
. After warming to 48 °C for 2 minutes, 200 µL of the hybridization solution is injected into cartridges housing the oligonucleotide arrays (Affymetrix GeneChip® 100K Mapping Set: 50K Array Xba 240 and 50K Array Hind 240). Hybridizations are carried out at 48 °C
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