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文献和实验Discovery and Characterization of Leucine-Rich Repeat-Containing G Protein-Coupled Receptors
Leucine-rich repeat-containing G protein-coupled receptors (LGRs) are type A rhodopsin-like GPCRs that include the glycoprotein pituitary hormone receptors for the thyroid-stimulating, follicle-stimulating, and luteinizing hormones (TSH, FSH
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
stock solution Nutrient-rich fly medium Phosphate-buffered saline (PBS; pH 7.5) Poly-L-lysine solution (0.1% [w/v] in H2 O; Sigma P8920) Sodium phosphate buffer (10 mM, pH 6.8) Triton X-100 VECTASTAIN Elite ABC Kit
Fastfilter Plasmid Midi Kit Spin Protocol
, DNA Wash Buffer must be brought to room temperature before use. 13. Optional Step: Repeat wash step with another 3.5 ml of DNA Wash Buffer. Centrifuge as above and discard fluid. 14. Centrifuge the empty capped column for 10-15 min
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