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文献和实验Methylated DNA Immunoprecipitation (MeDIP)
and quality of the sonication tip, therefore it is recommended to systematically check the size of the sheared DNA to ensure equal sonication between experiments. Dilute the genomic DNA in TE in a 1.5 ml eppendorf tube (10-20 µg DNA in 400 µl TE, 40
ELISA Procedure for Measuring Serum Antibody Titer
Dilution ScheduleDilution ofAntiserum Volume of 1% BSA/PBS-T (µL) Volume of PreviousDilution (µL) 1:100 1000 1000 of 1:50 1:200 1000 1000 of 1:100 1:400 1000 1000 of 1:200 1:800 1000 1000 of 1:400 ... ... ... 1:102,400 1000 1000 of 1:51,200 11. Vortex
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
at room temperature or overnight at 4°C with gentle shaking. Drain off excess solution. 27. Add 40 µL of diluted affinity-purified primary antibody to each slide, and cover with a coverslip. Incubate the slides for 1 h at room
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