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- 英文名：
Quick-RNA Plant Kit
- 库存：
大量
- 供应商：
简石生物
HIGHLIGHTS
- High Quality: Extract intact high quality total RNA (including small/micro RNAs) from a wide variety of plant samples.
- Homogenization: Ultra-high density BashingBead technology for complete disruption and sample lysis.
- Inhibitor-Free: RNA is ready for all downstream applications including sequencing, RT-PCR, microarray, etc.
DESCRIPTION
The Quick-RNA Plant Miniprep Kit provides for rapid isolation of RNA from various plant samples (e.g. leaves, stems, buds, flowers, fruit, seeds etc.). For purification of total RNA including small RNAs (~50 µg), the Quick-RNA Plant Miniprep features a specially formulated RNA Lysis Buffer. The Zymo-Spin IIICG Column allows for high-capacity DNA elimination and the subsequent Zymo-Spin IIC Column efficiently adsorbs total RNA. The RNA is washed and then eluted with DNase/RNase-Free Water. For inhibitor removal, the eluted RNA can be treated by running the sample through the Zymo-Spin III-HRC Spin Filters. The RNA is suitable for use in various subsequent procedures including RT-PCR.
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文献和实验实测植物总RNA提取液套装提取土豆、桑叶中的RNA 一直以来，搞植物研究的同学们对Trizol试剂提取植物RNA诟病不断，在这样的背景和需求下，我们特别研发了植物总RNA提取液套装，套装中除了植物总RNA提取液外，还包括了Buffer EX(氯仿替代品)，RNA沉淀液（异丙醇替代品）以及β-巯基乙醇。真可谓一个套装在手，提取植物RNA无烦恼！现在我们特别选择了土豆块茎（Trizol试剂提取失败）和桑叶（Trizol试剂提取效率极低）两个典型样本实测植物总RNA提取液套装的效果。 实验目的
植物RNA提取--Methods for Plant RNA Isolation
mL EtOH 6.0g SDS We homogenize the plant material(usually leaves) and dilute the sap 1:5 in AMES We then incubate for 30min. @37℃ followed by the addition of an equal vol.of chloroform.Vortex and store on ice. We then centrifuge the samples @ 3000rpm
A quick RNA mini-prep for Neurospora mycelial cultures
is thus desirable. Such a system has been developed for isola-ting plant RNA (Nagy et al. 1988 Plant Molecular Biology Manual, B4; ed. Gelvin and Schilperoort, Klewer Academic Publishing, pp. 1-29), and we have adapted this procedure for use with Neurospora
