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- 技术资料
- 英文名:
EZ DNA Methylation-Direct Kit
- 保质期:
1年
- 供应商:
简石生物
- 保存条件:
常温
HIGHLIGHTS
- Complete bisulfite conversion of DNA directly from blood, soft tissue, cells, FFPE samples, and LCM samples.
- Compatible with small sample inputs as few as 10 cells or 50 pg of DNA.
- DNA recovered by this bisulfite conversion kit is ideal for downstream analyses including PCR, endonuclease digestion, sequencing, microarrays, etc.
DESCRIPTION
The EZ DNA Methylation-Direct kit is a bisulfite conversion kit that features reliable and complete DNA bisulfite conversion directly from blood, tissue, and cells without the prerequisite for DNA purification. The increased sensitivity of this bisulfite conversion kit makes it possible to amplify bisulfite-converted DNA from as few as 10 cells or 50 pg DNA. Recovered bisulfite-converted DNA is ideal for downstream analyses including PCR amplification, endonuclease digestion, sequencing, microarrays, etc.
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文献和实验• 以96孔板方式,从同一个细胞或组织样本中同时纯化基因组 DNA和总RNA • 处理 96 个样本,只需不到60 分钟 • 从同一样本中纯化高品质的基因组DNA和总RNA • 96孔板方式,高效的操作流程 • 高度标准化的操作流程 • 高重复性的DNA和RNA产量 Product description The AllPrep DNA/RNA 96 Kit is well suited for high
, temperatures, and amount of template DNA, may vary and must be individually determined. 1) Thaw primer solutions. Keep on ice after complete thawing, and mix well before use. 2) Mix the PCR Master Mix by vortexing briefly. It is important
seeds c. Place a 4 mm stainless steel grinding ball in each well of the 2 ml 96 square well block and cover with sealing mat. Place block in the bead mill and shake at 1,500 rpm for 10 min. d. Continue to Extract seeds DNA.
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资料下载:
D5020-5021 甲基化 direct.pdf 附 (下载 35 次)
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