Bsp13I内切酶北京品牌

特别提示：包括Bsp13I内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：Bsp13I内切酶
英文名称：Bsp13I
产品货号：GS2146
产品规格：500U

储存条件：冷冻（-20℃）

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(dam-)in 1hourat 50℃ in a total reaction volume of 50μl.
Recognition sites	T↑CCGGA
来源	Bacillus species 13
Assayed on	Lambda DNA(dam-)
Optimal buffer	2K(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;200 mM KCl;1 mM DTT.)
Optimal temperature	50℃
Storage conditions	10mMTris-HCl(pH 7.5);200 mM KCl;0.1 mM EDTA;7 mM 2-mercaptoethanol;200 μg/ml BSA;50%glycerol;Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 20 u.a.of enzyme for 16hoursat 50℃.
Methlation effect	Blocked by overlapping Dam methylation(GmATC):TCCGGATC?and?GATCCGGA.
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	AccIII,Aor13HI,BlfI,BseAI,BsiMI,BspEI,BspMII,Kpn2I,MroI

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