DraIII内切酶北京供应商

特别提示：包括DraIII内切酶在内，本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！

产品名称：DraIII内切酶
英文名称：DraIII
产品货号：GS2209
产品规格：250U

储存条件：冷冻（-20℃）

使用说明

High enzyme concentration may result in star activity.To obtain 100%activity,BSA should be added to the 1 x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.?

技术规格
 

Unit definition	One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 37℃ in a total reaction volume of 50μl.
Recognition sites	CACNNN↑GTG
来源	E.coli strain,that carries the cloned gene Dra III from Deinococcus radiophilus
Assayed on	Lambda DNA
Optimal buffer	2K(10mMTris-HCl(pH 7.6 at 25℃);10mMMgCl2;200 mM KCl;1 mM DTT.)+ BSA
Optimal temperature	37℃
Storage conditions	10mMTris-HCl(pH 7.5);300 mM NaCl;0.1 mM EDTA;1 mM DTT;200 μg/ml BSA;50%glycerol.Store at -20℃.
Ligations	After 10-fold overdigestion with enzyme 70%of the DNA fragments can be ligated and recut.In the presence of 10%PEG ligation is better.
Non-specific hydrolisis	No nonspecific activity was detected after incubation of 1 μg of DNA with 10 u.a.of enzyme for 16hoursat 37℃.
Methlation effect	Methylation sensitivity not tested
Thermal inactivation	enzyme is inactivated by incubation at 65℃ for 20 mins.
Campatible Ends	AdeI

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